Plus end-directed microtubule motor required for chromosome congression
AssigneeThe Regents of the University of California
InventorsKenneth W. Wood, Roman Sakowicz, Lawrence S. B. Goldstein, Don W. Cleveland
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Abstract
The invention provides isolated nucleic acid and amino acid sequences of Xenopus CENP-E (XCENP-E), antibodies to XCENP-E, methods of screening for CENP-E modulators using biologically active CENP-E, and kits for screening for CENP-E modulators.
Claims5
An isolated biologically active centromere binding protein E wherein the centromere binding protein E (i) has plus end-directed microtubule motor activity, and (ii) comprises an amino acid sequence having at least 80% sequence identity with amino acid residues 1-324 of SEQ ID NO:1.
A kit for screening for modulators of centromere binding protein E, said kit comprising:
- i) a container holding biologically active centromere binding protein E wherein said biologically active centromere binding protein E comprises amino acid residues 1-324 of SEQ ID NO:1;
- and
- ii) instructions for assaying for centromere binding protein E activity, wherein the centromere binding protein E activity is plus end-directed microtubule motor activity.
An isolated biologically active centromere binding protein E, wherein said centromere binding protein E comprises amino acid residues 1-324 of SEQ ID NO:1.
Dependencies are inferred from the claim text.