US 6,645,745 B1 · Identification of a new member of the cytochrome P450 3A (CYP3A) gene family: CYP3AX

Abstract

The present invention relates to polynucleotides encoding the CYP3AX protein and variants thereof. Further, the present invention also provides vectors comprising said polynucleotides, in particular vectors, wherein polynucleotides of the present invention are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, the present invention relates to proteins encoded by said polynucleotides and antibodies specifically recognizing such proteins. The present invention also concerns transgenic non-human animals comprising the above-described polynucleotide or vectors. Moreover, the present invention relates to methods for identifying and obtaining drug candidates and inhibitors for therapy of disorders related to the malfunction of the CYP3AX genes as well as to methods of diagnosing the status of such disorders. The present invention also relates to methods for the identification of molecular variants of the CYP3AX polynucleotide or protein. The present invention furthermore provides pharmaceutical and diagnostic compositions comprising the above-described polynucleotides, vectors, proteins, antibodies, drugs and inhibitors obtainable by the above-described method. Said compositions are particularly useful for diagnosing and treating various diseases with drugs that are substrates, inhibitors or modulators of CYP3AX genes or their product.

Claims22

Dependent claims

An isolated polynucleotide encoding a cytochrome P450 (CYP) 3AX polypeptide that retains biological activity, selected from the group consisting of:

(a) a polynucleotide encoding a polypeptide comprising the amino acid sequence depicted in SEQ ID NO:
2, 4, 6, 8, 10 or 12;
(b) a polynucleotide encoding a polypeptide, said polynucleotide comprising a coding sequence as depicted in any one of SEQ ID NO:
1, 3, 5, 7, 9 or 11;
and
(c) a polynucleotide the nucleotide sequence of which is a variant of the nucleotide sequence of a polynucleotide of any one of (a) to (b), due to genetic code degeneracy;
provided that the polynucleotide does not consist of the nucleotide sequence set forth in SEQ ID NO:
35, in SEQ ID NO:
36, in SEQ ID NO:
37 or in SEQ ID NO:
38.

8 more elements

Dependencies are inferred from the claim text.

Identification of a new member of the cytochrome P450 3A (CYP3A) gene family: CYP3AX

Life of this patent

Identification of a new member of the cytochrome P450 3A (CYP3A) gene family: CYP3AX

Life of this patent

Abstract

Claims22