Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
AssigneePhoenix Pharmacologies, Inc.
InventorMike A. Clark
Life of this patent
Estimating the term…
Abstract
The present invention provides novel expression systems for producing desired polypeptides in certain strains of yeast. The present invention further provides methods for producing polypeptide products using such expression systems. The present invention also provides compositions relating to the same.
Claims13
An isolated nucleic acid molecule having promoter activity having the nucleotide sequence of SEQ ID NO:1.
A yeast-based expression system for the production of desired polypeptides, said expression system comprising a yeast host cell, wherein said yeast host cell is Komagataella pastoris, and an expression vector, said vector comprising a nucleic acid molecule encoding a desired polypeptide, which nucleic acid molecule is operably linked to one or more regulatory regions, and a promoter comprising the sequence of SEQ ID NO:1.
A method of expressing a desired polypeptide in a yeast host cell, wherein said yeast host cell is Komagataella pastoris, comprising transforming said yeast host cell with a vector comprising a promoter, wherein said promoter comprises SEQ ID NO:
- 1, and a nucleic acid sequence molecule encoding a desired polypeptide, thereby expressing said desired polypeptide.
Dependencies are inferred from the claim text.