Reagent for GPT assay
AssigneeNitto Boseki Co., Ltd.
InventorsYoshiro Sato, Chie Satokawa, Ryo Kojima, Katsuhiro Katayama
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Abstract
A two reagent-type kit for assaying GPT by acting GPT on L-alanine and α-ketoglutarate in the presence of pyridoxal phosphate, converting the resulting pyruvate into lactate with L-lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH) and measuring GPT based on a decrement of NADH, the GPT assay kit comprising a first reagent and a second reagent, one of which contains pyridoxal phosphate, L-alanine, α-ketoglutarate, LDH and NADH and the other reagent contains no pyridoxal phosphate but contains L-alanine. The GPT assay kit is stable over a long period of time.
Claims2
A two reagent kit for assaying glutamic-pyruvic transaminase (GPT) by reacting GPT with L-alanine and α-ketoglutarate in the presence of pyridoxal phosphate, converting the resulting pyruvate into lactate with L-lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH) and measuring GPT based on a decrease of NADH, the kit for assaying GPT:
- 1) comprising a first reagent and a second reagent, said first reagent and said second reagent being in liquid form, wherein:
- 2) the first reagent contains pyridoxal phosphate, LDH and NADH, and has a pH in the range of 8.5 to 9.0;
- and
- 3) the second reagent contains L-alanine and α-ketoglutarate, and has such a pH that a liquid prepared by mixing the second reagent with the first reagent to measure GPT will have pH in the range of 7 to 8.
Dependencies are inferred from the claim text.