Antigen-specific IgG detection
AssigneeRoche Diagnostics GmbH
InventorsElke Faatz, Urban Schmitt
Life of this patent
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Abstract
The invention concerns a method for the determination of antigen-specific antibodies of the immunoglobulin G class in the presence of immunoglobulins of the M class in body fluids by incubation with at least two different receptors R1 and R2 and optionally additional receptors, an essential component of R2 being a binding partner in monomeric form, a reagent for determining an antigen-specific antibody of the immunoglobulin G class as well as the use of binding partners in monomeric form for the determination of an antigen-specific antibody of the immunoglobulin G class.
Claims9
A method for the determination of an antigen-specific antibody of the immunoglobulin G class in a biological sample comprising the steps of:
- a. forming a mixture by combining said sample with at least two different receptors, R1 and R2, in which each of said receptors binds specifically to said antibody via its antigen-binding site according to the double antigen test principle, R1 is bound to a solid phase and R2 comprises a conjugate of a binding partner in monomeric form and a label, thereby forming a complex comprising solid phase-R1-antibody-R2-label, characterized in that the sample is combined together simultaneously with R1 and R2 in one step,
- b. separating the solid phase complex from the mixture, and
- c. measuring said label bound to said antibody via R2 as a measure of said antibody present in said sample.
Dependencies are inferred from the claim text.