Antibody compositions for preparing enriched mesenchymal progenitor preparations
AssigneeStemCell Technologies Inc.
InventorsTerry Thomas, Emer Clarke
Life of this patent
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Abstract
The present invention relates to antibody composition that are useful in preparing enriched mesenchymal progenitor cell preparations. The invention also relates to kits for carrying out the processes and to the cell preparations prepared by the processes.
Claims18
A negative selection process for enriching and recovering human mesenchymal progenitor cells in a sample containing human mesenchymal progenitor cells wherein said process comprises (1) reacting the sample with an antibody composition containing antibodies capable of binding to the antigens (a) CD2 and/or CD3 and/or CD5 and/or both CD4 and CD8;
- (b) CD66b and/or CD16 and/or OD11b and/or CD15;
- and (c) CD19 and/or CD20 and/or CD21 and/or CD22 and/or CD24 and/or Ig, under conditions so that conjugates are formed between the antibodies and cells in the sample containing the antigens (a) CD2 and/or CD3 and/or CD5 and/or both CD4 and CD8;
- (b) CD66b and/or CD16 and/or OD11b and/or CD15;
- and (c) CD19 and/or CD20 and/or CD21 and/or CD22 and/or CD24 and/or Ig, on their surfaces;
- (2) removing the conjugates;
- and (3) recovering a cell preparation which is enriched in human mesenchymal progenitor cells.
A negative selection method for enriching and recovering human mesenchymal progenitor cells in a sample containing the human mesenchymal progenitor cells and erythrocytes comprising:
- (1) contacting the sample with an antibody composition comprising (i) antibodies capable of binding to the antigens (a) CD2 and/or CD3 and/or CD5 and/or both CD4 and CD8;
- (b) CD66b and/or CD16 and/or CD11b and/or CD15;
- and (c) CD19 and/or CD20 and/or CD21 and/or CD22 and/or CD24 and/or Ig, wherein each of the antibodies in the antibody composition is linked to (ii) at least one antibody that binds to the erythrocytes, under conditions to allow immunorosettes of cells that bind to the antibodies in the antibody composition and the erythrocytes to form;
- and (2) separating the immunorosettes from the remainder of the sample to obtain a sample enriched in human mesenchymal progenitor cells.
A negative selection method for enriching and recovering human mesenchymal progenitor cells in a sample containing the human mesenchymal progenitor cells and erythrocytes comprising:
- (1) contacting the sample with an antibody composition comprising (i) antibodies capable of binding to the antigens (a) CD3;
- (b) CD66b;
- (c) CD19;
- (d) CD14 and (e) CD33 or CD38, wherein each of the antibodies in the antibody composition is linked to (ii) at least one antibody that binds to the erythrocytes, under conditions to allow immunorosettes of cells that bind to the antibodies in the antibody composition and the erythrocytes to form;
- and (2) separating the immunorosettes from the remainder of the sample to obtain a sample enriched in human mesenchymal progenitor cells.
A negative selection process for enriching and recovering human mesenchymal progenitor cells in a sample containing human mesenchymal progenitor cells wherein said process comprises (1) reacting the sample with an antibody composition containing antibodies capable of binding to the antigens (a) CD2 and/or CD3 and/or CD5 and/or both CD4 and CD8;
- (b) CD66b and/or CD16 and/or CD11b and/or CD15;
- and (c) CD19 and/or CD20 and/or CD21 and/or CD22 and/or CD24 and/or Ig;
- (d) CD14 and (e) CD33 and/or CD38, under conditions so that conjugates are formed between the antibodies and cells in the sample containing the antigens (a) CD2 and/or CD3 and/or CD5 and/or both CD4 and CD8;
- (b) CD66b and/or CD16 and/or CD11b and/or CD15;
- and (c) CD19 and/or CD20 and/or CD21 and/or CD22 and/or CD24 and/or Ig;
- (d) CD14 and (e) CD33 and/or CD38, on their surfaces;
- (2) removing the conjugates;
- and (3) recovering a cell preparation which is enriched in human mesenchymal progenitor cells.
A negative selection process for enriching and recovering human mesenchymal progenitor cells in a sample containing human mesenchymal progenitor cells wherein said process comprises (1) reacting the sample with an antibody composition containing antibodies capable of binding to the antigens (a) CD2 and/or CD3 and/or CD5 and/or both CD4 and CO8;
- (b) CD66b and/or CD16 b and/or CD11b and/or CD15;
- and (c) CD19 and/or CD20 and/or CD21 and/or CD22 and/or CD24 and/or Ig;
- (d) CD14;
- (e) CD33 and/or CD38;
- and (f) CD56, under conditions so that conjugates are formed between the antibodies and cells in the sample containing the antigens (a) CD2 and/or CD3 and/or CD5 and/or both CD4 and CD8;
- (b) CD66b and/or CD16 and/or CD11b and/or CD15;
- and (c) CD19 and/or CD20 and/or CD21 and/or CD22 and/or CD24 and/or Ig;
- (d) CD14;
- (e) CD33 and/or CD38;
- and (f) CD56, on their surfaces;
- (2) removing the conjugates;
- and (3) recovering a cell preparation which is enriched in human mesenchymal progenitor cells.
A negative selection method for enriching and recovering human mesenchymal progenitor cells in a sample containing the human mesenchymal progenitor cells and erythrocytes comprising:
- (1) contacting the sample with an antibody composition comprising (i) antibodies capable of binding to the antigens (a) CD3;
- (b) CD66b;
- (c) CD19;
- (d) CD14;
- (e) CD36;
- and (f) CD38, wherein each of the antibodies in the antibody composition is linked to (ii) at least one antibody that binds to the erythrocytes, under conditions to allow immunorosettes of cells that bind to the antibodies in the antibody composition and the erythrocytes to form;
- and (2) separating the immunorosettes from the remainder of the sample to obtain a sample enriched in human mesenchymal progenitor cells.
Dependencies are inferred from the claim text.