Diagnostic assay for endometriosis
AssigneeResearch Corporation Technologies, Inc.
InventorGrant R. Yeaman
Life of this patent
Estimating the term…
Abstract
The present invention provides a method for detecting endometriosis in a patient and is an improvement over invasive and expensive surgical procedures. The method employs immunoassays which detect autoantibodies in a serum sample which react with Thomsen-Friedenreich antigen (Tf). Increased levels of autoantibodies in a serum sample from the patient which bind to Tf-like antigen is indicative of endometriosis in the patient.
Claims18
A method for diagnosing endometriosis in a patient, said method comprising:
- (a) obtaining an antibody-containing tissue or an antibody-containing fluid sample from said patient,
- (b) incubating an antigen with said sample, wherein said antigen comprises a Galβ1-3GalNAc disaccharide moiety,
- (c) detecting autoantibody reactivity of said sample to said antigen, and;
- (d) correlating an increased level of autoantibody reactivity with a normal level to diagnose endometriosis in said patient.
A method for diagnosing endometriosis in a patient, said method comprising:
- (a) immobilizing an antigen on a solid support, wherein said antigen comprises a Galβ1-3GalNAc disaccharide moiety,
- (b) adding an aliquot of an antibody-containing fluid from a patient to said antigen to form a mixture and incubating the mixture, wherein said antibody binds to said antigen to form a complex,
- (c) adding anti-human immunoglobulins that are capable of detectably binding to said complex, wherein said anti-human immunoglobulins have a detectable label.
- (d) separating free anti-human immunoglobulins from bound anti-human immunoglobulins,
- (e) measuring the amount of bound anti-human immunoglobulins;
- and
- (f) correlating an increased amount of bound anti-human immunoglobulins with a normal amount of bound anti-human immunoglobulins to diagnose endometriosis in said patient.
A method for diagnosing endometriosis in a patient, said method comprising:
- (a) bringing together in an aqueous medium:
- (1) a predetermined amount of an antibody to an antigen comprising the Galβ1-3GalNAc disaccharide moiety, wherein said antibody has a detectable label, (2) an antigen which comprises a Galβ1-3GalNAc disaccharide moiety, and (3) an antibody-containing fluid sample from said patient,
- (b) separating labeled antibodies that are bound to said antigen from antibodies that are not bound to said antigen,
- (c) measuring the amount of labeled antibodies that are bound to said antigen, and;
- (d) correlating a decreased amount of labeled antibodies that are bound to said antigen with a normal amount of labeled antibodies that are bound to said antigen to diagnose endometriosis in said patient.
A method for diagnosing endometriosis in a patient, said method comprising:
- (a) preparing a reaction mixture by incubating a first antibody which is bound to an antigen with an antibody-containing fluid sample from a patient, wherein said antigen comprises a Galβ1-3GalNAc disaccharide moiety,
- (b) adding a predetermined amount of a second antibody to the reaction mixture wherein said second antibody recognizes the first antibody and wherein the second antibody has a detectable label,
- (c) separating free molecules of the second antibody from bound molecules of the second antibody,
- (d) measuring the signal generated by the bound molecules of the second antibody in the reaction mixture, and;
- (e) correlating a decreased level of signal with a normal level of signal to diagnose endometriosis in the patient.
Dependencies are inferred from the claim text.