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AssigneesConsejos Superior de Investigaciones Cientificas, Pharmacia Spain S.A.
InventorsCarlos Martinez Alonso, José Mario Mellado Garcia, Leonor Kremer Baron, José Miguel Rodriguez Frade
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The present invention relates to a method for determinating cytokine receptor, especially growth hormone receptor (GHR), activation, by the use of an antibody capable of discriminating between an activated and an inactive cytokine receptor conformation, especially GHR or growth hormone binding protein (GHBP) conformation, the method comprising: contacting the antibody and the cytokine receptor to form a complex, contacting a candidate ligand to the complex, measuring the antibody binding to inactive cytokine receptor and thereby discriminating between an activated and an inactive conformation. The invention also relates to an antibody directed to the hinge spanning subdomains I and II of the growth hormone receptor extracellular region and which is capable of discriminating between an activated and an inactive receptor conformation. The invention also relates to the use of the antibody for e.g. characterization of growth hormone receptor interactions and for screening for compounds stabilising die dimerization of GHR.
Method for determining growth hormone receptor activation by the use of GHR05 antibody, wherein the GHR05 antibody binds with a higher affinity to the active conformation of growth hormone receptor than it does to the inactive conformation of growth hormone receptor, the method comprising the steps of:
Method for determining growth hormone receptor activation by the use of GHR05 antibody, wherein the GHR05 antibody dissociates more slowly from the active conformation of a grow hormone receptor than it does from the inactive conformation of a growth hormone receptor, the method comprising the steps of:
The monoclonal antibody GHR05.
Dependencies are inferred from the claim text.