Detection of fungal pathogens using the polymerase chain reaction
AssigneeSyngenta Participations AG
InventorsCharles Jason Barnett, James Joseph Beck, Christy Violet Perry
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Abstract
The present invention relates to the use of primers in polymerase chain reaction assays for the detection of fungal pathogens Colletotrichum acutatum, Alternaria spp., and Cladosporium carpophilum. Specific primers are identified as being useful for the identification of fungal isolates using PCR based techniques. Also described are novel extraction buffer solutions for use in isolating DNA from an organism, methods of extracting DNA from tissue, and methods of performing PCR analysis on DNA extracted from tissue.
Claims9
An oligonucleotide primer consisting of SEQ ID Number 30.
A pair of oligonucleotide primers consisting of SEQ ID Number 30 and SEQ ID Number 4.
Dependencies are inferred from the claim text.