Solid medium and process for the storage and rapid purification of nucleic acid
AssigneeWhatman, Inc.
InventorsMartin A. Smith, Mridula Iyer, Daqing Qu, James C. Davis
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AssigneeWhatman, Inc.
InventorsMartin A. Smith, Mridula Iyer, Daqing Qu, James C. Davis
Estimating the term…
A medium for storage and subsequent analysis of a genetic material includes a support for immobilizing the genetic material thereon and allowing subsequent elution of the genetic material therefrom and a coating functionally associated with the support for enabling cellular lysis and releasing the genetic material from the lysed cells while stabilizing the immobilized released genetic material. A method of storing the genetic material and subsequently analyzing the genetic material includes the steps of immobilizing the genetic material on a support while enabling cellular lysis and release of genetic material from the lysed cells and stabilizing the immobilized released genetic material on the support. The genetic material is then eluted to generate a soluble genetic material fraction. The eluted genetic material can be analyzed.
A method of storing a genetic material and subsequently analyzing the genetic material by the steps of contacting cells having genetic material with a glass microfiber matrix sorbed with a FTA purification reagent while enabling cellular lysis and release of genetic material from the lysed cells, immobilizing and stabilizing the released genetic material, and disposing the matrix having the genetic material immobilized therein into heated water in the temperature range of 65° C. to 100° C. and releasing the genetic material into the heated water to generate a soluble genetic material fraction;
A method of storing a genetic material by contacting cells having genetic material with a glass microfiber matrix sorbed with a FTA purification reagent that lyses cells, releases the genetic material from the lysed cells, and allows subsequent heat elution of the genetic material into heated water in a temperature range of 65° C. to 100° C. while stabilizing the immobilized released genetic material.
Dependencies are inferred from the claim text.