Ionically formulated biomolecule microcarriers
AssigneeSedum Laboratories, Inc.
InventorJames E. Woiszwillo
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Abstract
Compositions for sustained delivery of a biomolecule including an anionic polymer and a cationic polymer which ironically interact with each other and, optionally, with the biomolecule. Methods for making the compositions, including the step of combining the negatively charged polymer with the positively charged polymer to form an ionic complex are also provided. The biomolecule may be complexed with one of the polymers before it is complexed with the oppositely charged polymer. The complex is exposed to conditions that cause the formation of precipitated microcarriers, such as a change in pH or the addition of a complexing molecule. The compositions are preferably formulated into microcarriers.
Claims15
A microparticle composition for delivery of a biomolecule comprising a biomolecule incorporated in a complex comprising a cationic polymer ionically complexed with an anionic polymer, wherein the cationic polymer is polyethyleneimine and the anionic polymer is dextran sulfate.
A method for producing microparticles comprising
- reacting a biomolecule, a cationic polymer, and an anionic polymer to form an ionically bonded complex of the polymers, wherein the biomolecule is incorporated in the polymer complex, the cation polymer is polyethyleneimine, and the anionic polymer is dextran sulfate, and
- precipitating the complex to form the microparticles.
A method for delivering a biomolecule to a human or animal subject comprising administering to the subject a microparticle composition comprising a biomolecule incorporated in a complex comprising a cationic polymer ionically complexed with an anionic polymer, wherein the microparticle releases the biomolecule in vivo, and wherein the cationic polymer is polyethyleneimine and the anionic polymer is dextran sulfate.
Dependencies are inferred from the claim text.