Anti-pathogen system and methods of use thereof
AssigneeWashington University
InventorSteven F. Dowdy
Life of this patent
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Abstract
The present invention provides an anti-pathogen system comprising one or more fusion proteins that includes a transduction domain and a cytotoxic domain. The cytotoxic domain is specifically activated by a pathogen infection. The anti-pathogen system effectively kills or injures cells infected by one or a combination of different pathogens. Further provided are protein transduction domains that provide enhanced transduction efficiency.
Claims27
A protein transduction domain comprising a peptide represented by the following formula:
- B1-X1-X2-X3-B2-X4-X5-B3 (SEQ ID NO:
- 42), wherein B1, B2, and B3 are each independently a basic amino acid, the same or different;
- and X1, X2, X3, X4 and X5 are each independently an alpha-helix enhancing amino acid the same or different, the protein transduction domain further comprising up to about 10 substantially aligned basic amino acid residues.
A protein transduction domain represented by the formula B1-X1—X2-X3-B2-X4-X5-B3 (SEQ ID NO:
- 42), wherein B1, B2, and B3 are each independently a basic amino acid, the same or different;
- and X1, X2, X3, X4 and X5 are each independently an alpha-helix enhancing amino acid the same or different.
A protein transduction domain comprising a peptide represented by the following formula:
- B1-X1-X2-B2-B3-X3-X4-B4 (SEQ ID NO:
- 43);
- wherein B1, B2, B3, and B4 are each independently a basic amino acid, the same or different;
- and X1, X2, X3, and X4 are each independently an alpha-helix enhancing amino acid the same or different, the protein transduction domain further comprising up to about 10 substantially aligned basic amino acid residues.
A protein transduction domain represented by the following formula:
- B1-X1-X2-B2-B3-X3-X4-B4 (SEQ ID NO:
- 43);
- wherein B1, B2, B3, and B4 are each independently a basic amino acid, the same or different;
- and X1, X2, X3, and X4 are each independently an alpha-helix enhancing amino acid the same or different.
A protein transduction domain comprising the following sequence:
- AGRKKRRQRRR (SEQ ID NO:
- 2), and up to about 10 substantially aligned basic amino acid residues.
A protein transduction domain comprising the following sequence:
- YARKARRQARR (SEQ ID NO. 3) and up to about 10 substantially aligned basic amino acid residues.
A protein transduction domain comprising the following sequence:
- YARAAARQARA (SEQ ID NO. 4) and up to about 10 substantially aligned basic amino acid residues.
A protein transduction domain comprising the following sequence:
- YARAARRAARR (SEQ ID NO. 5) and up to about 10 substantially aligned basic amino acid residues.
A protein transduction domain comprising the following sequence:
- YARAARRAARA (SEQ ID NO. 6) and up to about 10 substantially aligned basic amino acid residues.
A protein transduction domain comprising the following sequence:
- YARRRRRRRRR (SEQ ID NO. 7) and up to about 10 substantially aligned basic amino acid residues.
A protein transduction domain comprising the following sequence:
- YAAARRRRRRR (SEQ ID NO. 8) and up to about 10 substantially aligned basic amino acid residues.
A protein transduction comprising at least one of the following sequences:
- YARAAPRRR (SEQ ID NO:
- 56);
- YARAPRRARRR (SEQ ID NO:
- 37);
- YARAPRRPRRR (SEQ ID NO:
- 38);
- YARAAARPARA (SEQ ID NO:
- 39):
- YARAPARQARA (SEQ ID NO:
- 40);
- or YARAPARPARA (SEQ ID NO:
- 41).
Dependencies are inferred from the claim text.