Microencapsulated pheochromocyte of ox adrenal medulla as medicine for curing pain

A microencapsulated medicine of ox adrenal medulla pheochromocyte for treating pain, which is prepared through the following steps:

• (1) mixing the ox adrenal medulla pheochromocyte with 10-20 g/L solution of sodium alginate to form suspension, which contains 0.1-1×1010 cells per liter said suspension,
• (2) dispersing the suspension of step (1) into 80-120 mmol/L calcium chloride solution or calcium lactate solution in the state of microdrops of diameter of 150-1000 μm by spray device, the proportion of the two solution is to ensure 1 liter mixture to contain 0.1-1×108 cells, setting 5-20 minutes, removing the supernatant after depositing completely, then obtaining the calcium alginate bead deposit which contains ox adrenal medulla pheochromocytes;
• (3) adding the deposit derived from step (2) into the 0.3-0.7 g/L polylysine solution, the proportion of the two solution is to ensure 1 liter mixture contains 0.2-2×108 cells, mixing uniformly, setting 5-20 minutes, removing the supernatant after depositing completely, getting the deposit;
• (4) adding the deposit obtained from step (3) into 1.0-2.0 g/L sodium alginate solution, the proportion of the two solution is to ensure 1 liter mixture contains 0.2-2×108 cells, mixing it uniformly, setting 3-15 minutes, removing the supernatant after depositing completely, getting the deposit;
• (5) adding the deposit obtained from step (4) into 40-70 mmol/L sodium citrate solution, the proportion of the two solution is to ensure 1 liter mixture contains 0.2-2×108 cells, mixing it uniformly, setting 5-20 minutes, removing the supernatant after depositing completely, getting the microencapsulated pheochromocyte deposit of ox adrenal medulla;
• (6) washing the deposit obtained from step (5) by adding it into the 9 g/L soludium chloride solution, finally transferring the deposit into a cell culture, and storing it as microencapsulated medicine of ox adrenal medulla pheochromocyte.