Basidiomycete manganese peroxidase gene-transferred plant and a method for removing an environmental contaminant using the same
AssigneesAgency of Industrial Science and Technology, Yosuke Iimura
InventorsYosuke Iimura, Yoshihiro Katayama
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Abstract
The present invention relates to a plant into which DNA encoding a basidiomycete-derived peroxidase is transferred, said DNA being expressed therein, and to a method for decomposing and removing hazardous chemicals in an environment effectively by using the plant.
Claims18
A plant into which a DNA comprising the coding sequence of a basidiomycete manganese peroxidase has been transferred, wherein the basidiomycete manganese peroxidase comprises amino acids 27-365 of SEQ ID NO:2, and wherein the basidiomycete manganese peroxidase is expressed in the plant.
A method for removing an environmental contaminant from an environment comprising the contaminant, wherein the environmental contaminant can be decomposed by a basidiomycete manganese peroxidase, the method comprising:
- (a) transferring a DNA comprising the coding sequence of a basidiomycete manganese peroxidase into a plant, wherein the coding sequence of a basidiomycete manganese peroxidase is selected from:
- (i) a nucleotide sequence that encodes amino acids 27-365 of SEQ ID NO:2;
- (ii) a nucleotide sequence that comprises nucleotides 79-1095 of SEQ ID NO:1;
- (iii) a nucleotide sequence that encodes amino acids 1-365 of SEQ ID NO:2;
- and
- (iv) a nucleotide sequence that comprises nucleotides 1-1095 of SEQ ID NO:1;
- and
- (b) cultivating the plant into which the DNA comprising the coding sequence of a basidiomycete manganese peroxidase has been transferred in the environment comprising the environmental contaminant to decompose the environmental contaminant and thereby remove the environmental contaminant from the contaminated environment.
A method for removing an environmental contaminant from an environment comprising the contaminant, wherein the environmental contaminant can be decomposed by a basidiomycete manganese peroxidase, the method comprising:
- (a) transferring a DNA comprising the coding sequence of a basidiomycete manganese peroxidase into a plant, wherein the coding sequence of a basidiomycete manganese peroxidase is selected from:
- (i) a nucleotide sequence that encodes amino acids 27-365 of SEQ ID NO:2;
- (ii) a nucleotide sequence that comprises nucleotides 79-1095 of SEQ ID NO:1;
- (iii) a nucleotide sequence that encodes amino acids 1-365 of SEQ ID NO:2;
- and
- (iv) a nucleotide sequence that comprises nucleotides 1-1095 of SEQ ID NO:1, and wherein the basidiomycete manganese peroxidase exists in an active and stable form on the cell surface of the roots of the plant;
- and
- (b) cultivating the plant into which the DNA comprising the coding sequence of a basidiomycete manganese peroxidase has been transferred in the environment comprising the environmental contaminant to decompose the environmental contaminant and thereby remove the environmental contaminant from the contaminated environment.
An isolated plasmid that comprises a coding sequence of a basidiomycete manganese peroxidase, wherein the coding sequence is selected from:
- (a) a nucleotide sequence that encodes amino acids 27-365 of SEQ ID NO:2;
- (b) a nucleotide sequence that comprises nucleotides 79-1095 of SEQ ID NO:1;
- (c) a nucleotide sequence that encodes amino acids 1-365 of SEQ ID NO:2;
- and a nucleotide sequence that comprises nucleotides 1-1095 of SEQ ID NO:1.
Dependencies are inferred from the claim text.