Peptide ligands for the erythropoietin receptor
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsStephen J. McConnell, Dominic G. Spinella
Life of this patent
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Abstract
Clones isolated from phage display libraries that bound to an erythropoietin (EPO) receptor probe are disclosed. Peptides encoded by sequences of those clones that bound to the EPO receptor are disclosed. A 12-mer amino acid consensus sequence, CXXGWVGXCXXW (where X represents one of many amino acids), common to the peptides that bound to the EPO receptor, but unrelated to the primary structure of EPO, is disclosed.
Claims32
A isolated polypeptide capable of binding to a human erythropoietin receptor, said isolated polypeptide selected from the group consisting of:
- Xn1-Xn2-C-X1-X2-G-W-V-G-X3-C-X4-X5-W-Xc1-Xc2 SEQ ID NO. 88;
- Xn1-Xn2-Xn3-C-X1-X2-G-W-V-G-X3-C-X4-X5-W-Xc1-Xc2 SEQ ID NO. 89;
- and
- Xn1-Xn2-Xn3-Xn4-C-X1-X2-G-W-V-G-X3-C-X4-X5-W-Xc1-Xc2 SEQ ID NO. 90
- wherein,
- Xn1-Xn2-Xn3, or Xn1-Xn2-Xn3, or Xn1-Xn2-Xn3-Xn4 are N-terminal peptides selected from peptides of from 2 to 4 natural α-amino acids in length comprising Xn1-Xn2, or Xn1-Xn2-Xn3, or Xn1-Xn2-Xn3-Xn4, respectively;
- X1, X2, X3, X4 and X5 are independently selected from the group consisting of natural α-amino acids;
- and
- Xc1-Xc2 is a C-terminal dipeptide.
An isolated polypeptide having the amino acid sequence of SEQ ID NO:81.
An isolated polypeptide having the amino acid sequence of SEQ ID NO:82.
Dependencies are inferred from the claim text.