Crustacean antimicrobial peptides
AssigneesInstitut Francais de Recherche pour l'Exploitation de la Mer, Centre National De La Recherche Scientifique
InventorsDelphine Destoumieux, Evelyne Bachere, Philippe Bulet
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Abstract
The invention concerns antimicrobial peptides obtained from penaeid prawns having the following characteristics: a molecular mass of about 5 to 7 kDa; a pHi not less than 9; an N-terminal portion comprising a region (A) of about 15 to 25 amino acids rich in proline; and a C-terminal portion comprising a region (B) of about 20 to 30 amino acids and containing 6 cysteine residues forming three intramolecular disulfide bonds. The invention also concerns the nucleic acid sequences coding for said peptides and enabling their production by genetic engineering.
Claims9
A purified antimicrobial peptide obtained from penaeid prawns with a molecular mass of about 5 to 7 kDa and an isoelectric pH greater than or equal to 9;
- said peptide comprising:
- a) a N-terminal region (A) of about 15 to 25 amino acids, which comprises the following motif (I):
- ProXaa1 ProXaa2 ProXaa3 Pro(SEQ ID NO:15 or 19)
- in which Xaa1 is a nonpolar neutral amino acid, Xaa2 is a basic amino acid and Xaa3 is a proline or a peptide bond;
- and
- b) a C-terminal region (B) of about 20 to 30 amino acids, which contains 6 cysteine residues forming three intramolecular disulfide bridges and arranged according to the following motif (IV):
- Cys S1 Cys S2 Cys Cys S3 Cys Cys (SEQ ID NO:
- 16 or 20)
- in which S1 is an amino acid or a peptide sequence of 2 or 3 amino acids, S2 is a peptide sequence of 10 amino acids, and S3 is a peptide sequence of 5 amino acids.
A method of producing an antimicrobial peptide obtained from penaeid prawns with a molecular mass of about 5 to 7 kDa and an isoelectric pH greater than or equal to 9, wherein the peptide comprises:
- a) a N-terminal region (A) of about 15 to 25 amino acids, which comprises the following motif (I):
- Pro Xaa1 Pro Xaa2 Pro Xaa3 Pro (SEQ ID NO:15 or 19), in which Xaa1 is a nonpolar neutral amino acid, Xaa2 is a basic amino acid and Xaa3 is a proline or a peptide bond;
- and
- b) a C-terminal region (B) of about 20 to 30 amino acids, which contains 6 cysteine residues forming three intramolecular disulfide bridges and which comprises the following motif (IV):
- Cys S1 Cys S2 Cys Cys S3 Cys Cys (SEQ ID NO:
- 16 or 20), in which S1 is an amino acid or a peptide sequence of 2 or 3 amino acids, S2 is a peptide sequence of 10 amino acids and S3 is a peptide sequence of 5 amino acids, the method comprising:
- expressing a nucleic acid sequence encoding the peptide in a host cell transformed with a recombinant vector containing the nucleic sequence under the transcriptional control of an appropriate promoter, wherein the peptide is expressed in the host cell.
Dependencies are inferred from the claim text.