Multihued labels
AssigneeTrellis Bioinformatics, Inc.
InventorsLawrence M. Kauvar, John Sedat
Life of this patent
Estimating the term…
Abstract
Particulate labels that can be individually identified comprise particulate supports to which are bound at least two distinguishable signal generating moieties, such as fluorophores emitting at different wavelengths, which signals are detectable and measurable in situ. By varying the ratio and/or amounts of the signal generating moieties, a multiplicity of different and distinguishable labels is obtained. Each different label can then be coupled to a different reagent and the individual interactions of each reagent with a target observed in parallel.
Claims19
A spatially defined arrangement of particulate different labels wherein each different label comprises a particulate support to which is coupled at least two signal generating fluorophores wherein each of said fluorophoresemits a detectable signal different from that emitted by the other(s) and wherein the magnitude of each of said emitted signals is varied among said different labels
- whereby each different label is thereby characterized by a different hue, which hue is detected and distinguished from the other hues in the multiplicity of labels whereby each different label is identifiable in situ, and
- wherein said arrangement of particulate labels is displayed on a surface to permit determination of the spatial position of said labels by means of fluorescence microscopy;
- and
- wherein each different label is associated with a different reagent.
Dependencies are inferred from the claim text.