Genetic engineering of plant chloroplasts
AssigneeAuburn University
InventorsHenry Daniell, Bruce A. McFadden
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Abstract
The present invention provides a method for transforming plants with foreign DNA by introducing foreign DNA into chloroplasts. The invention provides cassettes which introduce genetic elements into chloroplast of target plants. The plants are transformed with the desired phenotype such as herbicide resistance or of other properties. Transformed plants are also disclosed.
Claims36
A stably transformed transcription/translation active chloroplast of a lower plant, which is competent for uptake of exogenous DNA, which comprises an expression cassette comprising exogenous DNA comprising a coding sequence, control sequences which comprise a promoter functional in chloroplast and a transcription termination sequence functional in chloroplast, whereby there is provided expression of the coding sequence in the chloroplast, and chloroplast DNA flanking the expression cassette to facilitate stable integration of the DNA with chloroplast DNA making up the genome of the cell by homologous recombination, whereby the DNA is stably integrated and inherited through organelle replication in daughter cells.
An expression cassette for stably transforming chloroplast of lower plants comprising exogenous DNA comprising a coding sequence, control sequences comprising a promoter functional in chloroplast and a transcription terminator sequence functional in chloroplast, and chloroplast DNA sequences flanking the expression cassette to facilitate stable integration of the DNA with chloroplast DNA making up the genome of the cell by homologous recombination, whereby the DNA is stably integrated and inherited through organelle replication in daughter cells.
A stably transformed transcription/translation active non-green plastid of a plant, which is competent for uptake of exogenous DNA, which comprises an expression cassette comprising exogenous DNA comprising a coding sequence, control sequences which comprise a promoter functional in non-green plastids and a transcription termination sequence functional in non-green plastids, whereby there is provided expression of the coding sequence in the non-green plastid, and plastid DNA flanking the expression cassette to facilitate stable integration of the exogenous DNA with plastid DNA making up the genome of the cell by homologous recombination, whereby the exogenous DNA is stably integrated and inherited through organelle replication in daughter cells.
An expression cassette for stably transforming non-green plastid of plants comprising exogenous DNA comprising a coding sequence, control sequences comprising a promoter functional in non-green plastid and a transcription terminator sequence functional in non-green plastid, and plastid DNA sequences flanking the expression cassette to facilitate stable integration of the DNA with plastid DNA making up the genome of the cell by homologous recombination, whereby the DNA is stably integrated and inherited through organelle replication in daughter cell.
Dependencies are inferred from the claim text.