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US 6,642,042 B2Granted
Producing viruses utilizing immortal avian cells
AssigneeMerial Limited
InventorsJean-François Bouquet, Catherine Cleuziat, Jacques Samarut, Philippe Desmettre
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Abstract
The invention features apoptosis-resistant, non-transformant immortalised avian cells, in particular, avian tissues, i.e., other than blood or haematopoietic cells, particularly fibroblasts and epithelial cells, for instance embryos.
Claims12
A method of producing viruses in an avian cell line, said method comprising:
- providing avian cells which are immortalized, but untransformed;
- inserting an anti-apoptotic bcl-2 gene into the genome of said avian cells;
- culturing said avian cells to
- express the anti-apoptotic bcl-2 gene so as to allow said avian cells to produce a virus or a viral peptide, protein, glycoprotein or protein molecule.
1 more element
A method for producing viruses in the avian cell line TCF4.10 which is deposited in the CNCM under reference number I-1710, said method comprising:
- providing avian cells from the cell line TCF4.10 which are immortalized, but untransformed;
- inserting a viral gene into the genome of said avian cells;
- culturing said avian cells to
- express the viral gene so as to allow said avian cells to produce a virus or a viral peptide, protein, glycoprotein or protein molecule.
1 more element
Dependencies are inferred from the claim text.