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AssigneeThe United States of America as represented by the Secretary of the Army
InventorsRichard K. Gordon, Bhupendra P. Doctor, Ashima Saxena, Shawn R. Feaster, Donald Maxwell, Michelle Ross, David Lenz, Keith Lejeune, Alan Russell
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An enzymatically active reusable sponge or foam, capable of regeneration with oximes, made of a polymer such as polyurethane is prepared for detoxification of hazardous compounds such as organophosphorus and organosulfur compounds. The foam or sponge contains a plurality of enzymes including enzymes selected from acetylcholinesterase, butyrylcholinesterase, triesterase, pseudocholinesterase, organophosphate hydrase, phosphotriesterase, paraoxonase and organophosphorus and organosulfur hydrolyzing enzymes. The sponge or foam may additionally contain activated carbon and an enzyme reactivation compound. A kit can be formed containing the sponge or foam and the compound for enzyme reactivation. The enzymatically active foam or sponge may be prepared using a two chamber device where enzymes and prepolymer are passed from separate chambers into a static mixing stator and are subjected to low shear mixing and extrusion to form by the sponge or foam.
A method of making an enzymatically active reusable polymeric sponge or foam, capable of regeneration with oximes, for the detoxification of a hazardous compound comprising immobilizing a plurality of enzymes on or within the sponge or foam by mixing the plurality of enzymes with a polyurethane prepolymer in an apparatus having a static mixing stator connected to both first chamber and a second chamber, wherein the first chamber contains a mixture of the plurality of enzymes and the second chamber contains the polyurethane prepolymer, and equal parts of the mixture of the plurality of enzymes and the polyurethane prepolymer are passed from the first and second chambers into the static mixing stator where the enzymes and prepolymer are mixed under low shear conditions while being rapidly and evenly extruded through the static mixing stator to form said sponge or foam, said plurality of enzymes capable of detoxifying organophosphorous and/or organosulfur compounds, wherein said plurality of enzymes comprises at least one enzyme selected from the group consisting of:
Dependencies are inferred from the claim text.