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US 6,642,036 B2Granted
Sinapoylglucose:malate sinapoyltransferase form malate conjugates from benozic acid glucosides
AssigneeE. I. Du Pont de Nemours and Company
InventorsDennis Flint, Knut Meyer, Paul Viitanen
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Abstract
A gene has been isolated from Arabidopsis encoding sinapoylglucose:malate sinapoyltransferase (SMT). SMT is responsible for the substitution of a glucose moiety on aromatic acid glucosides with a malate moiety in plant vacuoles. The enzyme is useful for the production of small molecules for materials manufacture.
Claims4
A method for the production of pHBA ester malate comprising:
- a) providing a host cell producing suitable levels of glycosylated pHBA;
- b) introducing into the host cell a nucleic acid molecule encoding sinapoylglucose:malate sinapoyltransferase selected from the group consisting of
- i.) An isolated nucleic acid molecule encoding a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:7;
- ii.) An isolated nucleic acid molecule encoding a polypeptide having at least 90% identity with an amino acid sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:7 and
- iii.) An isolated nucleic acid molecule that is complementary to 1 or 2
- wherein the sinapoylglucose:malate sinapoyltransferase catalyzes the substitution of a glucose moiety on the glycosylated pHBA with a malate moiety to form pHBA ester malate;
- and
- c) optionally recovering the pHBA ester malate.
5 more elements
Dependencies are inferred from the claim text.