Nucleic acids for detecting parvovirus and methods of using same
AssigneeV.I. Technologies, Inc.
InventorsAristides Lazo, Xiaojuan Zhao, Jodie Ann Tassello, Veronica Gibaja
Life of this patent
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Abstract
Disclosed are nucleic acids that can be used as probes for detecting parvovirus in a sample of nucleic acid molecules. The nucleic acids hybridize to regions encoding a non- structural protein or structural proteins of human parvovirus strain 19 or porcine parvovirus. Also disclosed are methods for detecting and amplifying parvovirus in a sample using the nucleic acids of the invention.
Claims24
A method of detecting a parvovirus nucleic acid molecule in a sample of nucleic acid molecules, the method comprising:
- providing a sample comprising a target nucleic acid molecule;
- contacting said sample with at least one member of a plurality of primer pairs under conditions that allow annealing of said at least one member to a homologous target nucleic acid molecule in said sample, thereby forming a plurality of first annealed primer-target nucleic acid molecule complexes, wherein said plurality of primer pairs includes
- a first pair comprising a first primer less than 31 nucleotides in length and comprising at least 15 nucleotides of SEQ ID NO:1 and a second primer less than 31 nucleotides in length and comprising at least 15 nucleotides of SEQ ID NO:2;
- a second pair comprising a third primer less than 31 nucleotides in length and comprising at least 15 nucleotides of SEQ ID NO:4, and a fourth primer less than 31 nucleotides in length and comprising at least 15 nucleotides of SEQ ID NO:5;
- extending said plurality of first annealed target nucleic acid molecule complexes with a polymerase to form a plurality of said first extended primer sequences;
- and
- identifying said plurality of extended primer sequences, thereby identifying a nucleic acid molecule in said sample of nucleic acid molecules.
A method for diagnosing the presence or susceptibility associated with a disease or condition associated with parvovirus infection in a subject, the method comprising
- providing a sample comprising a target nucleic acid molecule;
- contacting said sample with at least one member of a plurality of primer pairs under conditions that allow annealing of said at least one member to a homologous target nucleic acid molecule in said sample, thereby forming a plurality of first annealed primer-target nucleic acid molecule complexes, wherein said plurality of primer pairs includes
- a first pair comprising a first primer less than 31 nucleotides in length and comprising at least 15 nucleotides of SEQ ID NO:1 and a second primer less than 31 nucleotides in length and comprising at least 15 nucleotides of SEQ ID NO:2;
- a second pair comprising a third primer less than 31 nucleotides in length and comprising at least 15 nucleotides of SEQ ID NO:4, and a fourth primer less than 31 nucleotides in length and comprising at least 15 nucleotides of SEQ ID NO:5;
- extending said plurality of first annealed target nucleic acid molecule complexes with a polymerase to form a plurality of said first extended primer sequences;
- and
- identifying said plurality of extended primer sequences, wherein the identification of said plurality indicates that said subject has or susceptible to a disease or condition associated with parvoviris infection.
Dependencies are inferred from the claim text.