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AssigneeThe Immune Response Corporation
InventorsCharles R. Ill, Jose E. N. Gonzales, Claire Q. Yang, Scott Bidlingmaier
Estimating the term…
Novel genes and vectors exhibiting increased expression and novel splicing patterns are disclosed. The gene can comprise one or more consensus or near consensus splice sites which have been corrected. The gene can alternatively or additionally comprise one or more introns within coding or noncoding sequences. The gene can still further comprise modified 5′ and/or 3′ untranslated regions optimized to provide high levels and duration of tissue-specific expression. In one embodiment, the gene comprises the coding region of a full-length Factor VIII gene modified by adding an intron within the portion of the gene encoding the β-domain, so that the gene is expressed as a β-domain deleted Factor VIII protein. The novel Factor VIII gene can also be modified to correct one or more consensus or near consensus splice sites within or outside of the coding region.
An isolated DNA encoding a Factor VIII protein, wherein the DNA comprises one or more consensus or near consensus splice sites which have been corrected relative to the consensus or near consensus splice sites occurring in the factor VIII sequence of SEQ ID NO:
An isolated DNA comprising the coding region of a full-length Factor VIII gene, wherein the coding region contains an intron spanning the portion of the gene encoding the β-domain.
A method of increasing expression of a gene encoding a Factor VIII protein comprising correcting one or more consensus or near consensus splice sites within the nucleotide sequence of the gene and expressing the gene in a cell.
A method of increasing expression of a gene comprising identifying one or more consensus or near consensus splice sites within the gene using the following algorithm:
A method of increasing expression of a gene encoding a Factor VIII protein comprising identifying one or more consensus or near consensus splice sites within the gene using the following algorithm:
Dependencies are inferred from the claim text.