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US 6,642,027 B2Granted
Production of secreted polypeptides
AssigneeGenecor International, Inc.
InventorMaria R. Diaz-Torres
Life of this patent
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Abstract
Described herein are methods and compositions for the production and secretion of polypeptides. Included herein is the use of interrupting peptide transport activity for an increase in polypeptide production and/or secretion.
Claims16
A method of increasing secretion of a polypeptide in an Escherichia cell wherein said cell expresses at least one peptide transport protein, said method comprising:
- (a) inactivating at least one peptide transport protein in said cell, wherein said peptide transport protein is one of the gene products of the oligopeptide permease (opp) operon or the dipeptide permease (dpp) operon;
- and
- (b) culturing said cell under conditions suitable for expression and secretion of said polypeptide.
A method for producing a polypeptide in an Escherichia cell comprising the steps of:
- (a) obtaining an Escherichia cell comprising a nucleic acid encoding a polypeptide to be produced, said Escherichia cell further comprising at least one mutated peptide transport protein from the opp operon or the dpp operon, wherein said mutated peptide transport protein is inactivated;
- and
- (b) culturing said Escherichia cell under conditions suitable for expression such that said polypeptide is produced.
Dependencies are inferred from the claim text.