Guanylate-binding protein
AssigneeGenentech, Inc.
InventorDiane Pennica
Life of this patent
Estimating the term…
Abstract
A member of the guanylate-binding protein family, designated GBP-4, is provided. Also provided are isolated nucleic acid encoding GBP-4, vectors and host cells containing such nucleic acid molecule, and a method for producing the GBP-4 recombinantly.
Claims35
An isolated nucleic acid comprising DNA encoding a guanylate binding protein-4 (“GBP-4”) polypeptide comprising the sequence of amino acids 1 to 591 of FIG. 1 (SEQ ID NO:3).
An isolated nucleic acid comprising DNA encoding the same polypeptide encoded by the GBP-4 polypeptide cDNA in ATCC deposit no. 209,456.
An isolated nucleic acid comprising DNA which (a) hybridizes under stringent conditions with DNA encoding GBP-4 polypeptide comprising amino acids 1 to 591 of FIG. 1 (SEQ ID NO:
- 3), (b) encodes a GBP-4 polypeptide that binds to at least one guanine nucleotide, and (c) encodes a GBP-4 polypeptide comprising a Asp-Thr-Glu-Gly (amino acid residues 97-100 of SEQ ID NO:
- 3) GTP-binding consensus motif, wherein the stringent conditions are 0.015 M sodium chloride/0.0015 M sodium citrate/0.1% sodium dodecyl sulfate at 50° C.
An isolated nucleic acid comprising DNA (a) having at least 95% sequence identity to a DNA encoding a guanylate binding protein-4 (“GBP-4”) polypeptide comprising the sequence of amino acids 1 to 591 of FIG. 1 (SEQ ID NO:3) and (b) encoding a GBP-4 polypeptide that binds to at least one guanine nucleotide.
An isolated nucleic acid comprising DNA which (a) hybridizes under stringent conditions with DNA encoding GBP-4 polypeptide comprising amino acids 1 to 591 of FIG. 1 (SEQ ID NO:
- 3), (b) encodes a GBP-4 polypeptide that binds to at least one guanine nucleotide;
- and (c) encodes a GBP-4 polypeptide comprising:
- (i) a Asp-Thr-Glu-Gly (amino acid residues 97-100 of SEQ ID NO:
- 3) GTP-binding consensus motif;
- (ii) a Thr-Leu-Arg-Asp (amino acid residues 179-182 of SEQ ID NO:
- 3) potential casein kinase II phosphorylation site;
- (iii) a Ser-Gly-Lys-Glu (amio acid residues 568-571 of SEQ ID NO:
- 3) potential casein kinase II phosphorylation site;
- (iv) a Thr-Leu-Arg (amino acid residues 179-181 of SEQ ID NO:
- 3) potential protein kinase C phosphorylation site;
- (v) a Thr-Met-Arg (amino acid residues 562-564 of SEQ ID NO:
- 3) potential protein kinase C phosphorylation site;
- (vi) a Ser-Gly-Lys (amino acid residues 568-570 of SEQ ID NO:
- 3) potential protein kinase C phosphorylaton site;
- (vii) a Ser-Gln-Lys (amino acid residues 586-588 of SEQ ID NO:
- 3) potential protein kinase C phosphorylaton site;
- (viii) a Gly-Ile-Met-Val-Asn-Gly (amino acid residues 283-288 of SEQ ID NO:
- 3) potential N-myristoylation site;
- (ix) a Gly-Ser-Gln-Gln-Gly-Val (amino acid residues 579-584 of SEQ ID NO:
- 3) potential N-myristoylation site;
- or
- (x) a Cys-Phe-Ile-Ser (amino acid residues 554-557 of SEQ ID NO:
- 3) potential prenylation site, wherein the stringent conditions are 0.015 M sodium chloride/0.0015 M sodium citrate/0.1% sodium dodecyl sulfate at 50° C.
Dependencies are inferred from the claim text.