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US 6,642,022 B1Granted
Platelet-derived growth factor receptors
AssigneeThe Regents of the University of California
InventorsLewis Thomas Williams, Jaime A. Escobedo
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AssigneeThe Regents of the University of California
InventorsLewis Thomas Williams, Jaime A. Escobedo
Estimating the term…
DNA sequences encoding human platelet-derived growth factor receptors (hPDGF-R), and expression constructs comprising sequences which encode a receptor that can be secreted or incorporated into the membrane of a mammalian cell. Peptide fragments with functions equivalent to the wild-type receptor, conferring a PDGF-sensitive mitogenic response on cells lacking the receptor are provided. The constructs can be used for enhancing PDGF response of cells, determining the regions involved in transducing the signal in response to PDGF binding, providing mutated analogs and evaluating drugs for their physiologic activity. Soluble fragments comprising PDGF receptor sequences are also provided, including important intracellular kinase insert sequences which interact with intracellular proteins.
A purified and isolated recombinant nucleic acid encoding a type A human platelet-derived growth factor receptor polypeptide (A-hPDGF-R), or fragment thereof, wherein said fragment comprises at least about 13 amino acid residues, wherein the polypeptide has greater than 80% percent amino acid sequence identity to SEQ ID NO:4, and wherein the polypeptide has PDGF receptor activity.
A purified and isolated recombinant nucleic acid encoding a type B human platelet-derived growth factor receptor polypeptide (B-hPDGF-R) fragment, said fragment comprising at least about 50 contiguous amino acid residues of the B-hPDGF-R extracellular domain, wherein the fragment has greater than 80% percent amino acid sequence identity to SEQ ID NO:2, and wherein the polypeptide has PDGF receptor activity.
A composition comprising:
A purified and isolated recombinant nucleic acid encoding a type B human platelet-derived growth factor receptor polypeptide (B-hPDGF-R) having the sequence of SEQ ID NO:2.
A purified and isolated recombinant nucleic acid encoding a type A human platelet-derived growth factor receptor polypeptide (A-hPDGF-R) having the sequence of SEQ ID NO:4.
Dependencies are inferred from the claim text.