Method for inducing a response in one or more targeted cells
AssigneeOncosis LLC
InventorsManfred R. Koller, Bernhard O. Palsson, Timothy M. Eisfeld
Life of this patent
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Abstract
A method and apparatus is disclosed for selectively identifying, and targeting with an energy beam, specific cells within a mixed cell population, for the purpose of inducing a response in the targeted cells. Two or more fluorescent labels can be attached to the targeted cells, and distinguished from one another by a color camera. By use of this system, one can monitor and affect cells having a desired phenotype. For example, targeted cells can not only be inactivated by an energy beam, but can also be monitored through fluorescent labeling for changes in cell morphology, ion transport or other cellular functions.
Claims8
A method for inducing a response in one or more targeted cells within a biological specimen, comprising:
- illuminating a frame of cells in said biological specimen with a first wavelength of light, wherein said biological specimen has been treated with more than one label that, when excited by said first wavelength of light, emit energy at one or more different wavelengths;
- capturing an image of said frame of cells;
- determining the locations of one or more targeted cells within said image by reference to cells that are activated by said first wavelength of light;
- and
- steering an energy beam to said locations of said one or more targeted cells, wherein said energy beam is sufficient to induce a response in at least one of said one or more targeted cells.
A method for inducing a first response in a first targeted population of cells and a second response in a second targeted population of cells, comprising:
- illuminating said first targeted population of cells and said second targeted population of cells with a first wavelength of light;
- capturing an image comprising said illuminated first targeted population of cells and said illuminated second targeted population of cells;
- determining the locations of cells of said first targeted population of cells by reference to said image;
- determining the locations of said second targeted population of cells by reference to said image;
- and
- steering an energy beam to said locations of said cells of said first targeted population of cells and said cells of said second targeted population of cells in order to induce said first response in said cells of said first targeted population of cells and said second response in said cells of said second targeted population of cells.
Dependencies are inferred from the claim text.