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US 6,642,014 B1Granted
Enzyme activity screen with direct substrate replacement
AssigneeNovozymes A/S
InventorsHenrik Pedersen, Swen Hölder, Jørgen Kjems, Mette Lund
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Abstract
A method for in vitro selection, from a library of catalyst molecules, of a catalyst molecule of interest having a relatively more efficient specific catalytic activity of interest, as compared to the rest of the catalyst molecules within said library, and wherein said in vitro selection method is characterised by that it allows multiple catalytic activity turn-overs (i.e. substrate to product catalytic activity turn-overs), by the catalyst molecule of interest, before it is finally collected.
Claims9
A method for selecting a catalyst from a library of catalysts, comprising:
- a. providing a composition, said composition comprising
- (i) a library of catalysts comprising at least two different catalysts present in the library as individual units having the structure catalyst-linker-substrate, wherein the substrate and the catalyst are positioned in the unit in a configuration that allows a catalytic reaction to occur between the catalyst and the substrate so as to form at least one product that remains a part of the unit, thereby generating a unit having the structure catalyst-linker-product, and
- (ii) free substrate;
- wherein under suitable conditions the linker exchanges the product of the catalyst-linker-product unit with free substrate so as to regenerate the catalyst-linker-substrate unit;
- b. providing conditions suitable for catalysts in the library to catalyze the reaction of the substrate to form the at least one product, wherein at least one product of the catalytic reaction remains part of the unit and forms the catalyst-linker-product units;
- c. providing conditions suitable for an exchange to occur between the free substrate and a product in the structure catalyst-linker-product so as to regenerate the catalyst-linker-substrate units;
- d. repeating steps b and c at least once;
- and
- e. selecting a catalyst of interest.
6 more elements
Dependencies are inferred from the claim text.