Method for sequencing reaction cleanup by constant pressure differential ultrafiltration
AssigneeMillipore Corporation
InventorsJack T. Leonard, Constance MacDonald, Joseph Gabriels
Life of this patent
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Abstract
A method for sequencing reaction cleanup adapted to remove contaminants from a sequencing reaction, comprising the steps of: providing a defined quantity of sequencing reaction product; providing at least one ultrafiltration membrane having at least one surface; transferring the suspended sequencing reaction product to the surface of the ultrafiltration membrane; and applying a first constant pressure differential to the ultrafiltration membrane at a force capable of producing the sequencing reaction product substantially free of the contaminants.
Claims14
A method for reducing the level of contaminants in a sequencing reaction, comprising the steps of,
- providing a quantity of sequencing reaction product having a concentration of contaminants;
- transferring said sequencing reaction product to a plurality of wells of a multiwell plate, each said well including an ultrafiltration membrane;
- applying a first constant pressure differential to said sequencing reaction product in each said well at a force capable of causing said contaminants to pass through said ultrafiltration membrane while retaining said sequencing reaction product on said membrane as retentate;
- and
- recovering said retentate substantially free of said contaminants from said membrane.
A method of purifying biological material having a concentration of contaminants, comprising:
- suspending a finished sequencing reaction product in a solvent;
- transferring the suspension to an ultrafiltration membrane;
- applying a constant pressure differential to said suspension at a force sufficient to cause said contaminants to pass through said membrane;
- and
- recovering the resulting retentate.
Dependencies are inferred from the claim text.