US 6,641,995 B2Granted

Methods for identifying cell cycle regulators

AssigneeThe University Court of the University of Glasgow

InventorsSusanne M. Brown, Alasdair R. Maclean, June Harland

Life of this patent

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Abstract

A method is disclosed for identifying a substance capable of disrupting an interaction between (i) a herpes simplex virus (HSV) ICP34.5 polypeptide or a homologue thereof, or a derivative thereof, and (ii) proliferating cell nuclear antigen (PCNA) or a homologue thereof, or a derivative thereof, which method comprises: (a) providing an HSV ICP34.5 polypeptide or a homologue thereof, or a derivative thereof, as a first component; (b) providing PCNA, or a homologue thereof, or a derivative thereof, as a second component; (c) contacting the two components with a substance to be tested under conditions that would permit the two components to interact in the absence of the said substance; and (d) determining whether the said substance disrupts the interaction between the first and second components.

Claims5

Dependent claims

A method of producing a composition comprising a substance which inhibits the specific binding between (i) a herpes simplex virus (HSV) ICP34.5 polypeptide and (ii) proliferating cell nuclear antigen (PCNA), said substance being identified by a method comprising

(a) providing a first component selected from the group consisting of an HSV ICP34.5 polypeptide, the 63 amino acid C-terminus of ICP34.5, MyD116, and GADD34;
(b) providing PCNA as a second component;
(c) contacting the two components with a test substance under conditions that permit the two components to bind in the absence of said test substance;
(d) determining whether said test substance inhibits binding of the first and second components;
and
(e) mixing a test substance determined in step (d) to inhibit said binding of the first and second components with a carrier or diluent to produce said composition.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application: 09/750,099Filed 29 Dec 2000 · continuation
Priority: GB 970529914 Mar 1997
Published: US 2001/0001058 A110 May 2001
Granted: 4 Nov 2003
Term ends: Not estimatedThe source records don’t support an estimate.

Classification7 codes

G01N 33/57595
Involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins,…:…
C07K 14/4738
Not used: cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
G01N 33/56994
Viruses: herpetoviridae, e.g. cytomegalovirus, Epstein-Barr virus
G01N 2333/47
From vertebrates: assays involving proteins of known structure or function as defined in the subgroups

Family

PCT/GB98/00772Parent of this continuation · filed 16 Mar 1998
US 2001/0001058 A1Pre-grant publication · published 10 May 2001
Application09/380,760Parent of this continuation
US 6,235,467 B1Parent of this continuation
US 6,641,995 B2This patent

From the US filing history. Foreign family members aren’t listed.