Heat shock protein-based vaccines and immunotherapies
AssigneeSloan-Kettering Institute for Cancer Research
InventorsJames E. Rothman, F. Ulrich Hartl, Mee H. Hoe, Alan Houghton, Yoshizumi Takeuchi, Mark Mayhew
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Abstract
The present invention relates to methods and compositions for inducing an immune response in a subject, wherein the subject is administered an effective amount of at least one heat shock protein in combination with one or more defined target antigens. These methods and compositions may be used in the treatment of infectious diseases and cancers.
Claims38
A hybrid antigen comprising:
- (a) an antigenic domain of an infectious agent;
- and
- (b) a binding domain peptide that noncovalently binds to an hsp70 and comprises a heptameric region having the sequence motif HyXHyXHyXHy (SEQ ID NO:29) where Hy represents a hydrophobic amino acid and X is any amino acid;
- wherein the binding domain and the antigenic domain are covalently bound in vitro.
A hybrid antigen comprising:
- (a) an antigenic domain of an infectious agent covalently bound by a peptide bond to
- (b) a binding domain that comprises a peptide that noncovalently binds to an hsp70;
- wherein the binding domain and the antigenic domain are covalently bound in vitro.
A hybrid antigen comprising:
- (a) an antigenic domain of an infectious agent;
- and
- (b) a binding domain that comprises a peptide that noncovalently binds to an hsp70;
- wherein the binding domain and the antigenic domain are covalently bound in vitro;
- and
- wherein the hybrid antigen is not covalently bound to a heat shock protein.
A hybrid antigen consisting essentially of an antigenic domain of an infectious agent and a binding domain that non-covalently binds to an hsp70, wherein the antigenic domain is from a first source and the binding domain is from a second source different from the first source, and wherein the binding domain and the antigenic domain are covalently bound in vitro.
A hybrid antigen consisting essentially of an antigenic domain of an infectious agent, a binding domain that non-covalently binds to an hsp70, and a peptide linker separating the antigenic domain and the binding domain, wherein the antigenic domain is from a first source and the binding domain is from a second source different from the first source, and wherein the binding domain and the antigenic domain are covalently bound in vitro.
Dependencies are inferred from the claim text.