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US 6,639,657 B2Granted
Laser capture microdissection translation stage joystick
AssigneeArcturus Engineering, Inc.
InventorsThomas M. Baer, Mark A. Enright, David F. Head, Christopher E. Todd
Life of this patent
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Abstract
Systems and methods for laser capture microdissection are disclosed. An inverted microscope includes a translation stage joystick subsystem. The systems and methods provide the advantages of increased speed and much lower rates of contamination.
Claims8
A method, comprising:
- providing a sample that is to undergo laser capture microdissection;
- positioning said sample on a translation stage of a laser capture microdissection instrument and within an optical axis of said laser capture microdissection instrument;
- providing a transfer film carrier having a substrate surface and a laser capture microdissection transfer film coupled to said substrate surface;
- placing said laser capture microdissection transfer film in juxtaposition with said sample with a pressure sufficient to allow laser capture microdissection transfer of a portion of said sample to said laser capture micro dissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdisection film;
- and then
- moving said sample and said translation stage with a manual joystick subsystem that is connected to said translation stage;
- and then
- transferring a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdissection transfer film.
5 more elements
A method, comprising:
- providing a sample that is to undergo laser capture microdissection;
- positioning said sample on a translation stage of a laser capture microdissection instrument and within an optical axis of said laser capture microdissection instrument;
- providing a transfer film carrier having a substrate surface and a laser capture microdissection transfer film coupled to said substrate surface;
- placing said laser capture microdissection transfer film in juxtaposition with said sample with a pressure sufficient to allow laser capture microdissection transfer of a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdissection film;
- and
- moving said sample and said translation stage with a manual joystick subsystem that is connected to said translation stage;
- wherein moving said sample and said translation stage with said manual joystick subsystem includes reducing a scalar movement defined by an operator;
- adjusting a leverage ratio to control sample movement as a function of operator hand movement;
- and
- transferring a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdissection transfer film.
7 more elements
A method, comprising:
- providing a microscope having an optical path;
- providing a laser coupled to the microscope;
- the laser having a beam path;
- providing a translation stage coupled to the microscope;
- providing a sample that is to undergo laser microdissection;
- positioning said sample on the translation stage;
- moving the sample and the translation stage with a joystick that is connected to the translation stage;
- wherein moving the sample and the translation stage with the joystick includes reducing a scalar movement defined by an operator and adjusting a leverage ratio to control sample movement as a function of operator hand movement;
- providing a transfer film;
- positioning the transfer film in the beam path;
- transferring a portion of the sample to the transfer film.
8 more elements
Dependencies are inferred from the claim text.