Plant sterol reductases and uses thereof
AssigneeThe General Hospital Corporation
InventorsJyan-Chyun Jang, Jen Sheen
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Abstract
Disclosed are plant DNA molecules encoding C-14 sterol reductase polypeptides, as well as plant DNA molecules encoding polypeptides having at least 50% identity to the Arabidopsis C-14 sterol reductase; and vectors, cells and plants expressing such DNA. Also provided are methods for modifying the phenotype of a plant by expressing a C-14 sterol reductase DNA molecule in either sense or antisense orientations.
Claims28
A purified plant nucleic acid molecule that specifically hybridizes under highly stringent conditions to the complement of the sequence set forth in SEQ ID NO:2, wherein said highly stringent conditions comprise
- a) hybridization at 65° C. and 50% formamide,
- b) a first wash at 65° C., 2×SSC, and 1% SDS, and
- c) a second wash at 65° C. and 0.1% SDS, and 0.1×SSC, and said nucleic acid molecule encodes a protein having C-14 sterol reductase activity.
Dependencies are inferred from the claim text.