Compounds for protecting hydroxyls and methods for their use
AssigneeQuiatech AB
InventorMarek Kwiatkowski
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Abstract
A hydrocarbyldithiomethyl-modified compound of the Formula: R1—O—CH2—S—S—R2 or a salt thereof wherein R1 is an organic molecule and R2 is a hydrocarbyl is useful for protecting and/or blocking hydroxyl groups in organic molecules such as nucleotides. The hydrocarbyldithiomethyl-modified compounds can also be used for chemically synthesizing oligonucleotides and for sequencing nucleic acid compounds.
Claims26
A hydrocarbyldithiomethyl-modified compound comprising the formula:
- or a salt thereof, wherein
- R1 is an organic molecule;
- R1, R2, and R4 are together or separately H, hydrocarbyl, or a residue of a solid support;
- and
- said R2 comprises a fluorescent labeling group.
A hydrocarbyldithiomethyl-modified compound comprising the formula:
- or a salt thereof, wherein
- R1 is an organic molecule further comprising at least one hydroxyl group that is not hydrocarbyldithiomethyl-modified;
- and
- R2, R3, and R4 are together or separately H, hydrocarbyl, or a residue of a solid support.
A hydrocarbyldithiomethyl-modified compound comprising the formula:
- or a salt thereof,
- wherein said R1 is selected from the group consisting of modified or unmodified amino acids, peptides, proteins, carbohydrates, sterols, and steroids;
- and
- R2, R3, and R4 are together or separately H, hydrocarbyl, or a residue of a solid support.
A hydrocarbyldithiomethyl-modified compound comprising the Formula:
- R1—O—CH2—S—S—R2
- or a salt thereof, wherein
- R1 is an organic molecule selected from the group consisting of modified or unmodified amino acids, peptides, proteins, carbohydrates, sterols, and steroids;
- and
- R2 is a hydrocarbyl.
Dependencies are inferred from the claim text.