Method of dissolving preformed β-amyloid peptide fibrils in vitro by poly-lysine
AssigneeMai Dao
InventorKhue Vu Nguyen
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Abstract
Beta-amyloid peptide (βA) is a major fibrillar component of neuritic plaques in Alzheimer's disease (AD) brains and is related to the pathogenesis of the disease. The present invention provides a method using Poly-L-Lysine to dissolve preformed βA fibrils in vitro. Its efficiency is instantaneous. Poly-L-Lysine offers the simplest and most effective way to dissolve preformed βA fibrils. The method of this present invention can be used as a universal dissolver of all types of oligomeric β-sheet conformation, precursor of the fibrils. Poly-L-Lysine may also be useful as a future universal therapeutic agent to prevent and/or retard amyloidogenesis in vivo AD and other types of amyloid related disorders.
Claims1
A method of dissolving preformed β-amyloid peptide fibrils in vitro, comprising:
- (a) incubating β-amyloid peptides in water or in phosphate buffered saline at 37° C. for 5 days to form β-amyloid peptide fibrils;
- (b) contacting the preformed β-amyloid peptide fibrils with poly-L-lysine or poly-D-lysine;
- (c) evaluating the dissolving efficiency by negative-staining electron microscopy immediately.
Dependencies are inferred from the claim text.