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AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsNobuo Hanai, Motoo Yamasaki, Kenji Shibata, Akiko Furuya, Osamu Mikuni, Hideharu Anazawa
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The present invention provides a monoclonal antibody which can specifically and efficiently recognize hTERT protein; which is the catalytic subunit of telomerase, and provides a human chimeric antibody, a CDR grafted antibody, a single chain antibody, and a disulfide stabilized antibody each containing the monoclonal antibody. In addition, the present invention provides a method for detecting/quantitating hTERT protein using these antibodies, and provides diagnosis method, diagnosis agent, and therapeutic agent, for diseases, such as cancer, in which telomerase is involved using these bodies.
A monoclonal antibody, wherein said monoclonal antibody reacts specifically with the amino acid sequence of the human telomerase catalytic subunit, the sequence being designated as one of SEQ ID NOs:
A monoclonal antibody, wherein said monoclonal antibody is selected from the group consisting of monoclonal antibodies KM2311, KM2582, KM2590, KM2591, and KM2604.
A hybridoma which produces the monoclonal antibody wherein said hybridoma is selected from the group consisting of KM2311 (FERM-BP3603), KM2582 (FERM BP-6663), KM2590 (FERM 6683), KM2591 (FERM BP-6684), and KM2604 (FERM BP-6664).
A human chimeric antibody comprising an antibody heavy chain (H chain) variable region (V region) and an antibody light chain (L chain) V region of the monoclonal antibody, and H chain constant region (C region) and L chain C region of a human antibody, wherein amino acid sequences of said H chain V region and L chain V region have the same amino acid sequences as amino acid sequences of an H chain V region and an L chain V region of a monoclonal antibody selected from the group consisting of monoclonal antibodies KM2311, KM2582, KM2590, KM2591, and KM2604.
A CDR grafted antibody comprising V region complementary determining regions of H chain and L chain of the monoclonal antibody, and C region and V region framework regions of an H chain and an L chain of a human antibody, wherein amino acid sequences of said complementary determining regions of the H chain V region and L chain V region have the same amino acid sequences as amino acid sequence of complementary determining regions of an H chain V region and L chain V region of a monoclonal antibody which is selected from the group consisting of monoclonal antibodies KM2311, KM2582, KM2590 KM2591, and KM2604.
A disulfide stabilized antibody comprising H chain V region and L chain V region of monoclonal antibody, wherein amino acid sequences of an H chain V region and an L chain V region of said disulfide stabilized antibody have the same amino acid sequence as amino acid sequences of an H chain V region and an L chain V region of a monoclonal antibody which is selected from the group consisting of monoclonal antibodies KM2311, KM2582, KM2590, KM2591, and KM2604.
A disulfide stabilized antibody comprising H chain V region and L chain V region of monoclonal antibody, wherein amino acid sequences of an H chain V region and an L chain V region of said disulfide stabilized antibody have the same amino acid sequence as amino acid sequences of complementary determining regions of an H chain V region and an L chain V region of a monoclonal antibody which is selected from the group consisting of monoclonal antibodies KM 2311, KM2582, KM2590, KM2591, and KM2604.
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