Cloning and recombinant production of CFR receptor(s)

An immunogenic fragment of an isolated mammalian G protein-coupled corticotropin-releasing factor (CRF) receptor protein;

• wherein said protein is encoded by DNA that hybridizes under suitable stringency to the complement of polynucleotide sequences set forth in SEQ ID NO:1 or the nucleotide sequence of the CRF-R encoding portion of clone hctCRFR, deposited with the ATCC under accession number 75474, so as to allow identification of sequences having at least 50% nucleic acid identity with respect to the reference polynucleotide sequences;
• wherein said receptor protein binds CRF;
• and
• wherein said protein is at least about 70% pure (by weight of total proteins).

An isolated mammalian G protein-coupled corticotropin-releasing factor (CRF) receptor protein,

• wherein said protein is encoded by DNA that hybridizes to the complement of polynucleotide sequences set forth in SEQ ID NO:1 or the nucleotide sequence of the CRF-R encoding portion of clone hctCRFR, deposited with the ATCC under accession number 75474, under hybridization conditions comprising a temperature of about 42° C., a formamide concentration of about 20% and a salt concentration of about 0.6 M NaCl, followed by wash conditions comprising a temperature of about 42-50° C. and a salt concentration of about 0.3 M NaCl;
• wherein said receptor protein binds CRF;
• and
• wherein said protein is at least about 70% pure (by weight of total proteins).

An isolated mammalian G protein-coupled corticotropin-releasing factor (CRF) receptor protein,

• wherein said protein is encoded by DNA that hybridizes to the complement of polynucleotide sequences set forth in SEQ ID NO:1 or the nucleotide sequence of the CRF-R encoding portion of clone hctCRFR, deposited with the ATCC under accession number 75474, under hybridization conditions comprising a temperature of about 42° C., a formamide concentration of about 50%, and a salt concentration of about 5×SSPE, followed by wash conditions comprising a temperature of about 65° C. and a salt concentration of about 0.2×SSPE;
• wherein said receptor protein binds CRF;
• and
• wherein said protein is at least about 70% pure (by weight of total proteins).