Methods for delivering compounds into a cell

A method of expressing an exogenous nucleic acid sequence in a cell, said method comprising the steps of:

• delivering said exogenous nucleic acid sequence together with a halide compound into said cell;
• and
• applying ultrasound to said cell sufficient to facilitate expression of said exogenous nucleic acid sequence in said cell,
• wherein said halide compound is selected from the group consisting of 1-bromo-nonafluorobutane;
• perfluorooctyliodide;
• perfluorooctylbromide;
• 1-chloro-1-fluoro-1-bromomethane;
• 1,1,1-trichloro-2,2,2-trifluoroethane;
• 1,2-dichloro-2,2-difluoroethane;
• 1,1-dichloro-1,2-difluoroethane;
• 1,2-dichloro-1,1,3-trifluoropropane;
• 1-bromoperfluorobutane;
• 1-bromo-2,4-difluorobenzene;
• 2-iodo-1,1,1-trifluoroethane;
• 5-bromovaleryl chloride;
• 1,3-dichlorotetrafluoroacetone;
• bromine pentafluoride;
• 1-bromo-1,1,2,3,3,3-hexafluoropropane;
• 2-chloro 1,1,1,4,4,4-hexafluoro-2-butene;
• 2-chloropentafluoro-1,3-butadiene;
• iodotrifluoroethylene;
• 1,1,2-trifluoro-2-chloroethane;
• 1,2-difluorochloroethane;
• 1,1-difluoro-2-chloroethane;
• 1,1-dichlorofluoroethane;
• heptafluoro-2-iodopropane;
• bromotrifluoroethane;
• chlorotrifluoromethane;
• dichlorodifluoromethane;
• dibromofluoromethane;
• chloropentafluorethane;
• bromochlorodifluoromethane;
• dichloro-1,1,2,2-tetrafluoroethane;
• 1,1,1,3,3-pentafluoropentane;
• perfluorotributylamine;
• perfluorotripropylamine;
• 3-fluorobenzaldehyde;
• 2-fluoro-5-nitrotoluene;
• 3-fluorostyrene;
• 3,5-difluoroaniline;
• 2,2,2-trifluoroethylacrylate;
• 3-(trifluoromethoxy)-acetophenone;
• 1,1,2,2,3,3,4,4-octafluorobutane;
• 1,1,1,3,3-pentafluorobutane;
• 1-fluorobutane;
• 1,1,2,2,3,3,4,4-octafluorobutane;
• 1,1,1,3,3-pentafluorobutane;
• perfluoro-4 methylquinolizidine;
• perfluoro-N-methyl-decahydroquinone;
• perfluoro-N-methyl-decahydroisoquinone;
• perfluoro-N-cyclohexyl-pyrrolidine;
• tetradecaperfluoroheptane;
• dodecaperfluorocyclohexane;
• perfluoromethane;
• perfluoroethane;
• perfluoropane;
• perfluorobutane;
• perfluoropentane;
• perfluorohexane;
• perfluoroheptane;
• perfluorooctane;
• perfluorononane;
• perfluorodecane;
• perfluorododecane;
• perfluoro-2-methyl-2-pentene;
• perfluorocyclohexane;
• perflurodecalin;
• perfluorododecalin;
• perfluoropropylene;
• perfluorocyclobutane;
• perfluoro-2-butyne;
• perfluoro-2-butene;
• perfluorobuta-1,3-diene;
• perfluorobutylethyl ether;
• bis(perfluoroisopropyl) ether;
• bis(perfluoropropy) ether;
• perfluorotetrahydropyran;
• perfluoromethyl tetrahydrofuran;
• perfluoro t-butyl methyl ether;
• perfluoro isobutyl methyl ether;
• perfluoro n-butyl methyl ether;
• perfluoro isopropyl ethyl ether;
• perfluoro n-propyl ethyl ether;
• perfluoro cyclobutyl methyl ether;
• perfluoro cyclopropyl ethyl ether;
• perfluoro isopropyl methyl ether;
• perfluoro n-propyl methyl ether;
• perfluoro diethyl ether;
• perfluoro cyclopropyl methyl ether;
• perfluoro methyl ethyl ether;
• perfluoro dimethyl ether;
• sulfur hexafluoride;
• and selenium hexafluoride;
• wherein said delivering and said applying steps are conducted concurrently.

A method of expressing an exogenous nucleic acid sequence in a cell, the method comprising the steps of:

• delivering said exogenous nucleic acid sequence together with a halide compound into said cell;
• and
• applying ultrasound to said cell sufficient to express said exogeneous nucleic acid sequence in said cell,
• wherein said delivering step is preceded by said applying step.

A method of expressing an exogenous nucleic acid sequence in a cell, said method comprising the steps of:

• delivering said exogenous nucleic acid sequence together with a halide compound into said cell;
• and
• applying ultrasound to said cell sufficient to facilitate expression of said exogenous nucleic acid sequence in said cell,
• wherein said halide compound is selected from the group consisting of 1-bromo-nonafluorobutane;
• perfluorooctyliodide;
• perfluorooctylbromide;
• 1-chloro-1-fluoro-1-bromomethane;
• 1,1,1-trichloro-2,2,2-trifluoroethane;
• 1,2-dichloro-2,2-difluoroethane;
• 1,1-dichloro-1,2-difluoroethane;
• 1,2-dichloro-1,1,3-trifluoropropane;
• 1-bromoperfluorobutane;
• 1-bromo-2,4-difluorobenzene;
• 2-iodo-1,1,1-trifluoroethane;
• 5-bromovaleryl chloride;
• 1,3-dichlorotetrafluoroacetone;
• bromine pentafluoride;
• 1-bromo-1,1,2,3,3,3-hexafluoropropane;
• 2-chloro 1,1,1,4,4,4-hexafluoro-2-butene;
• 2-chloropentafluoro-1,3-butadiene;
• iodotrifluoroethylene;
• 1,1,2-trifluoro-2-chloroethane;
• 1,2-difluorochloroethane;
• 1,1-difluoro-2-chloroethane;
• 1,1-dichlorofluoroethane;
• heptafluoro-2-iodopropane;
• bromotrifluoromethane;
• chlorotrifluoromethane;
• dichlorodifluoromethane;
• dibromofluoromethane;
• chloropentafluoroethane;
• bromochlorodifluoromethane;
• dichloro-1,1,2,2-tetrafluoroethane;
• 1,1,1,3,3-pentafluoropentane;
• perfluorotributylamine;
• perfluorotripropylamine;
• 3-fluorobenzaldehyde;
• 2-fluoro-5-nitrotoluene;
• 3-fluorostyrene;
• 3,5-difluoroaniline;
• 2,2,2-trifluoroethylacrylate;
• 3-(trifluoromethoxy)-acetophenone;
• 1,1,2,2,3,3,4,4-octafluorobutane;
• 1,1,1,3,3-pentafluorobutane;
• 1-fluorobutane;
• 1,1,2,2,3,3,4,4-octafluorobutane;
• 1,1,1,3,3-pentafluorobutane;
• perfluoro-4 methylquinolizidine;
• perfluoro-N-methyl-decahydroquinone;
• perfluoro-N-methyl-decahydroisoquinone;
• perfluoro-N-cyclohexyl-pyrrolidine;
• tetradecaperfluoroheptane;
• dodecaperfluorocyclohexane;
• perfluoromethane;
• perfluorethane;
• perfluoropropane;
• perfluorobutane;
• perfluoropentane;
• perfluorohexane;
• perfluoroheptane;
• perfluorooctane;
• perfluorononane;
• perfluorodecane;
• perfluorodecane;
• perfluoro-2-methyl-2-pentene;
• perfluorocyclohexane;
• perfluorodecalin;
• perfluorododecalin;
• perfluoropropylene;
• perfluorocyclobutane;
• perfluoro-2-butyne;
• perfluoro-2-butene;
• perfluorobuta-1,3-diene;
• perfluorobutyethyl ether;
• bis(perfluoroisopropyl) ether;
• bis(perfluoropropyl) ether;
• perfluorotetrahydropyran;
• perfluoromethyl tetrahydrofuran;
• perfluoro t-butyl methyl ether;
• perfluoro isobutyl methyl ether;
• perfluoro n-butyl methyl ether;
• perfluoro isopropyl ethyl ether;
• perfluoro n-propyl ethyl ether;
• perfluoro cyclobutyl methyl ether;
• perfluoro cyclopropyl ethyl ether;
• perfluoro isopropyl methyl ether;
• perfluoro n-propyl methyl ether;
• perfluoro diethyl ether;
• perfluoro cyclopropyl methyl ether;
• perfluoro methyl ethyl ether;
• perfluoro dimethyl ether;
• sulfur hexafluoride;
• and selenium hexafluoride;
• wherein said delivering step is preceded by said applying step.