Loading patent…
AssigneePE Corporation (NY)
InventorsJer-Kang Chen, Claudia Chiesa, George A. Fry, Vergine C. Furniss, Stephen M. Lambert, Roger O'Neill, Majid Mehrpouyan
Estimating the term…
The present invention provides substrates and apparatuses for efficient, rapid and specific capture, and optimal recovery, of nucleic acids, as well as methods of their use. The substrate is porous in nature and has a capture polynucleotide capable of hybridizing to a target nucleic acid immobilized thereon. Upon flowing a sample containing or suspected of containing the target nucleic acid through the porous substrate, the target nucleic acid is rapidly captured. Following capture, the target nucleic acid can be efficiently recovered for subsequent use.
A flow-through device for capturing a target nucleic acid comprising a three-dimensional porous substrate composed of a polymeric material selected from the group consisting of polyethylene, polystyrene, polycarbonate and polypropylene and having immobilized thereon about 6×10−17 to 6×10−16 nmol/nm2 of a capture polynucleotide which is capable of hybridizing to the target nucleic acid, and wherein said porous substrate is about 1 mm to 20 mm thick.
The flow-through device of claim 1 or 6 in which the capture polynucleotide is covalently immobilized on the porous substrate via its 5′- or 3′-terminal residue.
A flow-through device for capturing a target nucleic acid, comprising a three-dimensional porous substrate composed of a polymeric material selected from the group consisting of polyethylene, polystyrene, polycarbonate and polypropylene and having an average pore size of about 10 μm to about 100 μm and a porosity in the range of about 25 to 80% and having immobilized thereon a capture polynucleotide capable of hybridizing to the target nucleic acid.
A flow-through device for capturing a target nucleic acid, comprising a three-dimensional porous substrate composed of a polymeric material selected from the group consisting of polyethylene, polystyrene, polycarbonate and polypropylene and having a porosity in the range of about 25 to 80% and having immobilized thereon a capture polynucleotide capable of hybridizing to the target nucleic acid.
A flow-through device for capturing a target nucleic acid, comprising a three-dimensional porous substrate composed of a polymeric material selected from the group consisting of glass, polyethylene, polystyrene, polycarbonate and polypropylene and having an average pore size of about 10 μm to about 100 μm and a porosity in the range of about 25 to 80% and having immobilized thereon about 6×10−17 to 6×10−16 nmol/nm2 of a capture polynucleotide which is capable of hybridizing to the target nucleic acid.
A flow-through device for capturing a target nucleic acid, comprising a three-dimensional porous substrate composed of a polymeric material selected from the group consisting of glass, polyethylene, polystyrene, polycarbonate and polypropylene and having an average pore size of about 10 μm to about 100 μm and a porosity in the range of about 25 to 80% and having immobilized thereon a capture polynucleotide capable of hybridizing to the target nucleic acid, wherein said porous substrate is about 1 mm to 20 mm thick.
A flow-through device for capturing a target nucleic acid, comprising a three-dimensional porous substrate composed of a polymeric material selected from the group consisting of glass, polyethylene, polystyrene, polycarbonate and polypropylene and having an average pore size of about 10 μm to about 100 μm and having immobilized thereon about 6×10−17 to 6×10−16 nmol/nm2 of a capture polynucleotide which is capable of hybridizing to the target nucleic acid, wherein said porous substrate is about 1 mm to 20 mm thick.
A flow-through device for capturing a target nucleic acid, comprising a three-dimensional porous substrate composed of a polymeric material selected from the group consisting of glass, polyethylene, polystyrene, polycarbonate and polypropylene and having a porosity in the range of about 25 to 80% and having immobilized thereon about 6×10−17 to 6×10−16 nmol/nm2 of a capture polynucleotide which is capable of hybridizing to the target nucleic acid, wherein said porous substrate is about 1 mm to 20 mm thick.
A flow-through device for capturing a target nucleic acid, comprising a three-dimensional porous substrate composed of a polymeric material selected from the group consisting of glass, polyethylene, polystyrene, polycarbonate and polypropylene and having an average pore size of about 1 μm to about 250 μm and a porosity in the range of about 25 to 80% and having immobilized thereon about 2×10−19 to 2×10−15 nmol/nm2 of a capture polynucleotide which is capable of hybridizing to the target nucleic acid, wherein said porous substrate is about 1 mm to 20 mm thick.
Dependencies are inferred from the claim text.