Process for the enzymatic resolution of lactams

A method of separating enantiomeric lactam esters, wherein the lactams are of the formula (V):

• R1 is selected from the group consisting of alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, which may optionally be substituted by one or more of the following:
• alkyl, alkenyl, alkynyl, hydroxy, alkoxy, thiol, thioalkoxy, and halogen;
• and
• R2, R3, and R4 are independently selected from alkyl, alkenyl, alkynyl, hydroxy, alkoxy, thiol, thioalkoxy, halogen, nitro, amino, alkylamino, dialkylamino, aminoalkyl, dialkylaminoalkyl, cyano, and haloalkyl, wherein all said substitutions may be optionally substituted with one or more of the following:
• halogen, alkyl, amino, alkylamino, dialkylamino, aminoalkyl, hydroxy, and alkoxy, comprising contacting the lactam esters with a biocatalyst in an aqueous solution, an organic solvent, or a mixture of organic and aqueous solvents wherein only one enantiomer is selectively hydrolyzed to give the optically active isomer of the corresponding acid, and separating the hydrolysis product from the unreacted lactam esters.