Simple culture medium and method for preparation thereof
AssigneeNissui Pharmaceutical Co., Ltd.
InventorsShingo Mizuochi, Hidemasa Kodaka, Hideaki Shibata
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Abstract
A simple culture medium produced by impregnating a fibrous water-absorbent sheet with a suspension formed by suspending in an alcohol (a) an adhesive (0.01-0.4 wt. %) which is soluble in both water and alcohol, (b) a gelling agent which is soluble in water and insoluble in alcohol, and (c) a bacterial nutritive ingredient, the fibrous water-absorbent sheet having a mesh larger than the particle size of the gelling agent and being placed on a waterproof flat plate, and by drying the resultant sheet while suppressing rapid evaporation of the alcohol, to thereby cause the water-absorbent sheet to adhere onto the waterproof flat plate; and a method for producing the medium.
Claims22
A simple culture medium, comprising (a) an adhesive, (b) a gelling agent, (c) a bacterial nutritive ingredient, a fibrous water-absorbent sheet, and a waterproof flat plate, produced by a process comprising:
- preparing an alcohol suspension by suspending in an alcohol (a) an adhesive, which is soluble in both water and alcohol, and is a hydroxypropyl cellulose, at a final concentration of 0.01 to 0.4% w/v (b) a gelling agent, which is soluble in water and insoluble in alcohol, and (c) a bacterial nutritive ingredient;
- impregnating a fibrous water-absorbent sheet with the alcohol suspension, wherein the fibrous water-absorbent sheet has a mesh larger than the particle size of the gelling agent and being placed on a waterproof flat plate;
- and
- drying the resultant sheet while suppressing rapid evaporation of alcohol, to thereby cause the water-absorbent sheet to adhere onto the waterproof flat plate.
A method for producing a simple culture medium, comprising preparing an alcohol suspension by suspending in an alcohol (a) an adhesive, which is soluble in both water and alcohol, and is a hydroxypropyl cellulose, at a final concentration of 0.01 to 0.4% w/v (b) a gelling agent, which is soluble in water and insoluble in alcohol, and (c) a bacterial nutritive ingredient;
- impregnating a fibrous water-absorbent sheet with the alcohol suspension, wherein the fibrous water-absorbent sheet has a mesh larger than the particle size of the gelling agent and wherein the water-absorbent sheet is placed on a waterproof flat plate;
- and
- drying the resultant sheet while suppressing rapid evaporation of alcohol, to thereby cause the water-absorbent sheet to adhere onto the waterproof flat plate.
Dependencies are inferred from the claim text.