Nucleotide sequences which code for the cma gene
AssigneesDegussa AG, Forschungszentrum Julich GmbH
InventorsMadhavan Nampoothiri K., Bettina Möckel, Lothar Eggeling, Hermann Sahm
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Abstract
The invention relates to a genetically modified coryneform bacterium, the cma gene of which is amplified, and an isolated polynucleotide which codes for cyclopropane-mycolic acid synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the cma gene in the bacteria and the use of the polynucleotide as a primer or hydridization probe.
Claims21
An isolated polynucleotide which encodes a protein comprising the amino acid sequence of SEQ ID NO:
- 2, wherein said protein has cyclopropane-mycolic acid synthase activity.
An isolated polynucleotide comprising nucleotides 248 to 1600 of SEQ ID NO:
- 1 which encodes a cyclopropane-mycolic acid synthase.
An isolated polynucleotide comprising SEQ ID NO:
- 1.
An isolated polynucleotide consisting of SEQ ID NO:
- 1 or fragment thereof which encodes a protein consisting of the amino acid sequence of SEQ ID NO:
- 2.
An isolated polynucleotide comprising the complement of SEQ ID NO:
- 1.
An isolated polynucleotide comprising a nucleotide sequence that is at least 70% identical to the polynucleotide of SEQ ID NO:
- 1, wherein said polynucleotide encodes a protein comprising the amino acid sequence of SEQ ID NO:
- 2, and wherein said protein has cyclopropane-mycolic acid synthase activity.
An isolated polynucleotide consisting of at least 15 consecutive nucleotides selected from one of SEQ ID NO:
- 1 or a complement of SEQ ID NO:
- 1.
A pJC1cma vector deposited under DSM13248.
A transformed bacterium comprising an isolated polynueleotide which encodes a protein comprising the amino acid sequence of SEQ ID NO:
- 2, wherein said protein has cyclopropane-mycolic acid synthase activity.
A Corynebacterium glutamicum DSM5715/pJC1cma deposited under DSM 13248.
Dependencies are inferred from the claim text.