Peptidyl prolyl cis-trans isomerases
AssigneeDanisco A/S
InventorsPatrick M. F. Derkx, Susan M. Madrid
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Abstract
The present invention provides a method for producing a secretable polypeptide in a host cell. In the method, a peptidyl prolyl cis-trans isomerase is overexpressed in a host cell, thereby increasing the yield of the secreted polypeptide.
Claims18
A method for producing a secretable polypeptide in a host cell, comprising over-expressing a peptidyl prolyl cis-trans isomerase in the host cell, thereby increasing the yield of the secreted polypeptide, wherein the peptidyl prolyl cis-trans isomerase comprises a signal sequence at its N-terminus and an endoplasmic reticulum retention signal at its C-terminus.
A method for producing a secretable polypeptide in a host cell, comprising overexpressing a peptidyl prolyl cis-trans isomerase in the host cell, thereby increasing the yield of the secreted polypeptide, wherein the peptidyl prolyl cis-trans isomerase is a polypeptide:
- (1) possessing foldase activity, (2) having a capability to catalyze the cis-trans isomerisation of a peptide bond on the N terminal side of proline residues in polypeptides, (3) having a signal sequence at the N-terminus, (4) having an endoplasmic reticulum retention signal at the C-terminus, (5) having a molecular weight of 20.7 kDa, and (6) having a deduced isoelectric point of 6.27.
A nucleic acid vector encoding a polypeptide comprising the amino acid sequence shown in SEQ ID NO:
- 5 or an amino acid sequence having at least 75% homology with SEQ ID NO:
- 5.
A method for producing a secretable polypeptide in a host cell, comprising overexpressing a peptidyl prolyl cis-trans isomerase in the host cell, thereby increasing the yield of the secreted polypeptide, wherein the peptidyl prolyl cis-trans isomerase is a polypeptide possessing foldase activity having a capability to catalyse the cis-trans isomerisation of a peptide bond on the N terminal side of proline residues in polypeptides, said peptidyl prolyl cis-trans isomerase being encoded by a nucleic acid capable of hybridising under high stringency conditions with a 20 base oligonucleotide derived from SEQ ID No:
- 2.
A method for producing a secretable polypeptide in a host cell, comprising overexpressing a peptidyl prolyl cis-trans isomerase in the host cell, thereby increasing the yield of the secreted polypeptide, wherein the peptidyl prolyl cis-trans isomerase is a polypeptide possessing foldase activity having a capability to catalyse the cis-trans isomerisation of a peptide bond on the N terminal side of proline residues in polypeptides, said peptidyl prolyl cis-trans isomerase being encoded by a nucleic acid capable of hybridising under high stringency conditions with a 17 base oligonucleotide derived from SEQ ID No:
- 1.
A method for producing a secretable polypeptide in a host cell, comprising overexpressing a peptidyl prolyl cis-trans isomerase in the host cell, thereby increasing the yield of the secreted polypeptide, wherein the peptidyl prolyl cis-trans isomerase is a polypeptide possessing foldase activity having a capability to catalyse the cis-trans isomerisation of a peptide bond on the N terminal side of proline residues in polypeptides, said peptidyl prolyl cis-trans isomerase being encoded by a nucleic acid which is at least 80% homologous to SEQ ID No:
- 2.
A method for producing a secretable polypeptide in a host cell, comprising overexpressing a peptidyl prolyl cis-trans isomerase in the host cell, thereby increasing the yield of the secreted polypeptide, wherein the peptidyl prolyl cis-trans isomerase is a polypeptide comprising the amino acid sequence shown in SEQ ID No:
- 5 or an amino acid sequence having at least 75% homology with SEQ ID No:
- 5.
A nucleic acid vector comprising a nucleic acid comprising the nucleotide sequence shown as SEQ ID No:
- 2 or a nucleotide sequence having at least 80% homology to SEQ ID No:
- 2.
Dependencies are inferred from the claim text.