Human protein kinases and uses therefor
AssigneeMillennium Pharmaceuticals, Inc.
InventorsRachel Meyers, Rosana Kapeller-Libermann, Mark Williamson
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Abstract
The invention relates to novel kinase nucleic acid sequences and proteins. Also provided are vectors, host cells, and recombinant methods for making and using the novel molecules.
Claims32
A method for identifying a compound which binds to a polypeptide, said method comprising contacting a polypeptide, or a cell expressing a polypeptide, with a test compound, and determining whether the polypeptide binds to the test compound;
- wherein said polypeptide is selected from the group consisting of:
- a) the polypeptide having the amino acid sequence set forth in SEQ ID NO:14, wherein said polypeptide has protein kinase activity;
- b) a polypeptide comprising a fragment of at least 15 contiguous amino acids of SEQ ID NO:14, wherein said polypeptide has protein kinase activity;
- c) a polypeptide encoded by a nucleic acid molecule which hybridizes to the complement of the sequence set forth in SEQ ID NO:13 or 15 under stringent conditions comprising hybridization in 6× sodium chloride/sodium citrate at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 50° C. to 65° C., wherein said polypeptide has protein kinase activity;
- and,
- d) a polypeptide having at least 75% sequence identity to the amino acid sequence of SEQ ID NO:14, wherein said polypeptide has protein kinase activity.
A method for identifying a compound which modulates the activity of a polypeptide, said method comprising:
- a) providing the polypeptide having the amino acid sequence set forth in SEQ ID NO:14, wherein said polypeptide has protein kinase activity;
- b) contacting said polypeptide with a test compound;
- and,
- c) determining the effect of the test compound on the activity of the polypeptide to thereby identify a compound which modulates the activity of the polypeptide.
A method for identifying a compound which modulates the activity of a polypeptide, said method comprising:
- a) providing a polypeptide comprising a fragment of at least 15 contiguous amino acids of SEQ ID NO:14, wherein said polypeptide has protein kinase activity;
- b) contacting said polypeptide with a test compound;
- and,
- c) determining the effect of the test compound on the activity of the polypeptide to thereby identify a compound which modulates the activity of the polypeptide.
A method for identifying a compound which modulates the activity of a polypeptide, said method comprising:
- a) providing a polypeptide encoded by a nucleic acid molecule which hybridizes to the complement of the sequence set forth in SEQ ID NO:13 or 15 under stringent conditions comprising hybridization in 6× sodium chloride/sodium citrate at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 50° C. to 65° C., wherein said polypeptide has protein kinase activity;
- b) contacting said polypeptide with a test compound;
- and,
- c) determining the effect of the test compound on the activity of the polypeptide to thereby identify a compound which modulates the activity of the polypeptide.
A method for identifying a compound which modulates the activity of a polypeptide comprising:
- a) providing a polypeptide having at least 75% sequence identity to the amino acid sequence of SEQ ID NO:14, wherein said polypeptide has protein kinase activity;
- b) contacting said polypeptide with a test compound;
- and,
- c) determining the effect of the test compound on the activity of the polypeptide to thereby identify a compound which modulates the activity of the polypeptide.
Dependencies are inferred from the claim text.