Methods and compositions for extended and super-extended DNA and hybridization mapping
AssigneeCTRC Research Foundation
InventorBradford E. Windle
Life of this patent
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Abstract
The present invention relates to preparation and methods of use of novel extended and super-extended forms of DNA. These forms of DNA are stretched to a substantially linear form up to about 0.6 μm per kilobase pair. The extended DNA may be hybridized with suitably labelled probes and visualized directly. Gene order and distance are rapidly and efficiently determined with a resolution down to 0.4 kb using fluorescent hybridization techniques.
Claims14
A method of producing super-extended DNA, comprising the steps:
- streaming a DNA sample over a supporting surface to provide a DNA extended to an interkilobase pair distance that exceeds about 0.34 μm.
A super-extended DNA that has an interkilobase pair distance greater than about 0.34 μm.
A super-extended DNA prepared by gravitationally streaming the DNA over a supporting surface wherein the DNA is stretched to an interkilobase pair distance of greater than about 0.34 μm.
Dependencies are inferred from the claim text.