Streptomyces megasporus sd5, process for the isolation thereof, novel fibrinolytic enzyme prepared therefrom, process for the production of said enzyme and method of treatment of thrombolytic disorders using said enzyme
AssigneeMaharashtra Association for the Cultivation of Science
InventorsRatnakar Ravindra Chitte, Sabita Dey
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Abstract
The present invention relates to a novel microorganism Streptomyces megasporus SD5. The present invention also relates to a process for the isolation of said Streptomyces megasporus SD5. The invention also relates to a novel fibrinolytic enzyme actinokinase extracted from said microorganism and to a process for the extraction of said enzyme. In another aspect, the invention also pertains to a method for the treatment of thrombolytic disorders using said enzyme.
Claims36
An enzyme extracted and isolated from the microorganism Streptomyces megasporus SD5 or mutant thereof which is isolated by a process which comprises the steps of:
- a) cultivating the microorganism Streptomyces megasporus SD5 or mutant thereof under aerobic conditions at a temperature in the range of 45° C. to 65° C. in an alkaline aqueous nutrient medium and
- b) recovering the enzyme.
A process for isolating an enzyme from the microorganism Streptomyces megasporus SD5 comprising cultivating the microorganism Streptomyces megasporus SD5 or mutant thereof under aerobic conditions at a temperature in the range of 45° C. to 65° C. in an alkaline aqueous nutrient medium and recovering the enzyme.
An enzyme extracted and isolated from the microorganism Streptomyces megasporous SD5 which is a serine protease of 264 amino acids and has a molecular weight of about 35 kDa as determined by SDS-PAGE.
An enzyme extracted from the microorganism Streptomyces megasporous SD5 prepared by the process which comprises the steps of:
- a) cultivating the microorganism Streptomyces megasporus SD5 under aerobic conditions at a temperature in the range of 45° C. to 65° C. in an alkaline aqueous nutrient medium and
- b) recovering the enzyme.
Dependencies are inferred from the claim text.