Methods for the separation of isomers
AssigneeBiostore New Zealand Limited
InventorPhillipa M. Wiggins
Life of this patent
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Abstract
Methods for the separation of chaotropic and kosmotropic enantiomers within a racemic mixture are provided. Such methods comprise differentially partitioning the enantiomers into stabilized microdomains of low density water and high density water abutting a porous surface.
Claims19
A method of separating at least two enantiomers in a racemic mixture, the method comprising differentially partitioning the enantiomers into regions of low density water and high density water abutting a porous surface, wherein one of the enantiomers is chaotropic and the other of the enantiomers is kosmotropic, and wherein the chaotropic enantiomer is selected from the group consisting of:
- D-glucose and L-amino acids;
- and the kosmotropic enantiomer is selected from the group consisting of:
- L-glucose and D-amino acids.
A method for separating a racemic mixture of a kosmotropic enantiomer and a chaotropic enantiomer, the method comprising:
- (a) providing a matrix of small-pored beads comprising pores of less than 3 nm in diameter;
- (b) stabilizing microdomains of high density water and low density water within the matrix, whereby the osmotic pressure in the high density microdomains is equal to that in the low density microdomains;
- (c) contacting the stabilized matrix with the mixture of enantiomers;
- and
- (d) breaking down low density water in the matrix whereby the chaotropic enantiomer is eluted from the gel.
A method of separating at least two enantiomers in a racemic mixture, the method comprising differentially partitioning the enantiomers into regions of low density water and high density water abutting a porous surface, wherein one of the enantiomers is chaotropic and the other of the enantiomers is kosmotropic, and wherein the separation of enantiomers is accomplished by:
- (a) balancing a concentration of kosmotropes in a solution external to pores within the porous surface with a concentration of chaotropes in a solution contained within the pores within the porous surface, whereby the regions of low and high density water are stabilized and wherein the osmotic pressure within the pores is equal to that external to the pores;
- (b) contacting the porous surface with the racemic mixture of enantiomers for sufficient time for partitioning to occur, wherein the chaotropic enantiomer partitions into low density water and the kosmotropic enantiomer partitions into high density water;
- and
- (c) removing the solution external to the pores, wherein the chaotropic enantiomer is retained selectively in low density water within the pores.
A method of separating at least two enantiomers in a racemic mixture, the method comprising differentially partitioning the enantiomers into regions of low density water and high density water abutting a porous surface, wherein one of the enantiomers is chaotropic and the other of the enantiomers is kosmotropic, and wherein the separation of enantiomers is accomplished by:
- (a) balancing the concentration of kosmotropes in a solution external to pores within the porous surface with the concentration of chaotropes in a solution contained within the pores within the porous surface, whereby the osmotic pressure within the pores is equal to that external to the pores;
- (b) contacting the porous surface with the racemic mixture of enantiomers to be separated for sufficient time for partitioning to occur, wherein the chaotropic enantiomer partitions into low density water and the kosmotropic enantiomer partitions into high density water;
- (c) removing the chaotropic enantiomer by converting low density water to high density water;
- and
- (d) removing the kosmotropic enantiomer by converting high density water to low density water.
A method for separating a racemic mixture of a kosmotropic enantiomer and a chaotropic enantiomer, the method comprising:
- (a) providing a matrix of small-pored beads comprising pores of less than 3 nm in diameter;
- (b) stabilizing microdomains of high density water and low density water within the matrix, whereby the osmotic pressure in the high density microdomains is equal to that in the low density microdomains;
- (c) contacting the stabilized matrix with the mixture of enantiomers;
- (d) contacting the matrix with a solution that breaks down low density water in the matrix whereby the chaotropic enantiomer is eluted from the gel;
- and
- (e) contacting the matrix with a solution that stabilizes low density water in the matrix, whereby the kosmotropic enantiomer is eluted from the matrix.
Dependencies are inferred from the claim text.