Methods for raising pre-adult anadromous fish
AssigneeMariCal, Inc.
InventorsWilliam Harris, Jr., David R. Russell, Jacqueling Noaring, Marlies Betka
Life of this patent
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Abstract
The invention relates to methods, compositions and kits for improving the raising of pre-adult anadromous fish, or preparing pre-adult anadromous fish for transfer to seawater. The methods involve adding PVCR modulators (e.g., calcium and/or magnesium) to the freshwater in an amount sufficient to increase expression and/or sensitivity of at least one PVCR; and adding feed for fish consumption to the freshwater, wherein the feed contains an amount of NaCl sufficient to contribute to a significantly increased level of the PVCR modulator in serum of the pre-adult anadromous fish.
Claims8
A method of determining whether a pre-adult anadromous fish is ready for transfer to seawater, wherein the pre-adult anadromous fish is subjected to a freshwater environment having a concentration of calcium in an amount between about 2.0 mM and about 10.0 mM and a concentration of magnesium in an amount between about 0.5 mM and about 10.0 mM, and feed having at least about 1% NaCl by weight;
- said method comprising assessing the amount of PVCR expression in the pre-adult anadromous fish, wherein an increased level of expression, as compared to a control, indicates that the pre-adult anadromous fish is ready for transfer to seawater.
The method of claim 2, further comprising:
- (a) contacting an anti-PVCR antibody to a sample comprising gill, skin, intestine, kidney, brain or muscle;
- under conditions sufficient for the formation of a complex between the antibody and the PVCR;
- and
- (b) detecting the formation of the complex.
A kit for determining whether a pre-adult anadromous fish is ready for transfer to seawater, wherein the pre-adult anadromous fish is subjected to a freshwater environment having magnesium and calcium, and feed having at least about 1% NaCl by weight, said kit comprises an anti-PVCR antibody.
A kit for determining whether a pre-adult anadromous fish is ready for transfer to seawater, wherein the pre-adult anadromous fish is subjected to a freshwater environment having magnesium and calcium, and feed having at least about 1% NaCl by weight, said kit comprises a nucleic acid sequence having a detectable label that can hybridize to nucleic acid of an aquatic PVCR.
A method of determining whether a salmon is ready for transfer to seawater, wherein the salmon is subjected to a freshwater environment having a concentration of calcium in an amount between about 2.0 mM and about 10.0 mM and a concentration of magnesium in an amount between about 0.5 mM and about 10.0 mM, and feed having at least about 1% NaCl by weight;
- said method comprising assessing the amount of PVCR expression in the salmon, wherein an increased level of expression, as compared to a control, indicates that the salmon is ready for transfer to seawater.
Dependencies are inferred from the claim text.