Methods and DNA constructs for gene silencing in transgenic plants
AssigneePlant Bioscience Limited
InventorsDavid Charles Baulcombe, Susan Mary Angell
Life of this patent
Estimating the term…
Abstract
The invention presents DNA constructs comprising a promoter operably linked to DNA which can be transcribed in a plant cell to an RNA transcript, wherein the RNA transcript comprises plant virus sequence from an RNA virus which confers on the RNA transcript the ability to replicate in the cytoplasm of the plant cell, wherein the transcript lacks all or part of the viral genome not required for replication in the cytoplasm, and further comprises at least one targeting sequence which is foreign to the plant virus sequence and causes post-transcriptional gene silencing of one or more target genes. The invention also presents methods to use the DNA construct to cause post-transcriptional gene silencing of a target gene in a plant.
Claims19
A recombinant DNA construct comprising a promoter operably linked to DNA which can be transcribed in a plant cell to an RNA transcript, which RNA transcript comprises plant virus sequence from an RNA virus which confers on the RNA transcript the ability to replicate in the cytoplasm of the plant cell wherein the RNA transcript lacks all or part of the sequence of said virus not required for replication in the cytoplasm and wherein the RNA transcript comprises at least one targeting sequence which causes post-transcriptional gene silencing of at least one target gene, wherein the targeting sequence is a sequence foreign to the plant virus sequence, and is 23 nucleotides or longer.
A recombinant Agrobacterium binary vector comprising a DNA construct comprising a promoter operably linked to DNA which can be transcribed in a plant cell to an RNA transcript, which RNA transcript comprises plant virus sequence from an RNA virus which confers on the RNA transcript the ability to replicate in the cytoplasm of the plant cell, said RNA transcript lacking all or part of the sequence of said virus not required for replication in the cytoplasm and wherein the RNA transcript comprises at least one targeting sequence which causes post-transcriptional gene silencing of a target gene wherein said at least one targeting sequence is a sequence foreign to the plant virus sequence and is 23 nucleotides or longer.
A plant host cell comprising a recombinant DNA construct comprising a promoter operably linked to DNA which can be transcribed in said plant cell to an RNA transcript, which RNA transcript comprises plant virus sequence from an RNA virus which confers on the RNA transcript the ability to replicate in the cytoplasm of the plant cell, said transcript lacking all or part of the sequence of said virus not required for replication in the cytoplasm and wherein the RNA transcript comprises at least one targeting sequence which causes post-transcriptional gene silencing of a target gene, wherein the targeting sequence is a sequence foreign to the plant virus sequence, and is 23 nucleotides or longer.
Dependencies are inferred from the claim text.