Process for extracting glycoside using an aqueous two-phase system
AssigneeIndustrial Technology Research Institute
InventorsI-Hong Pan, Chu-Hsun Lu, Hsi-Ho Chiu, Lain-Tze Lee, Hsin-Jan Yao
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Abstract
An aqueous two-phase system for extracting glycosides from a herb includes the extractive solution, about 5 wt %-30 wt % of salt, about 5 wt %-30 wt % of polyol and selectively about 0 wt %-30 wt % of alcohols. The salt can be dihydrogen phosphate, hydrogen phosphate and phosphate or a mixture thereof, and it also can be sulfates, chlorides, oxalates, or acetates. In addition, a process for extracting glycosides using an aqueous two-phase system is disclosed. The procedures includes preparation of extractive solution, concentration, phase separation, drying, washing with solvent, cooling, filtration and drying of the final products.
Claims37
A process of extracting glycosides from a plant, comprising the steps of:
- (a) grinding the plant and mixing with water to form an extractive solution;
- (b) adding about 5 wt %˜30 wt % of salt and about 5 wt %˜30 wt % of a polyol comprising a copolymer of ethylene oxide and propylene oxide, to the extractive solution and then mixing thoroughly to form an aqueous layer and a polyol layer of the copolymer of ethylene oxide and propylene oxide;
- (c) removing the aqueous layer and concentrating the aqueous layer to be a solid;
- (d) washing the solid with a solvent, and producing a filtrate and collecting the filtrate after filtration;
- and
- (e) concentrating the filtrate to obtain a final product rich in glycosides.
An aqueous two-phases system for extracting glycosides from a plant, comprising:
- an extractive solution;
- about 5 wt %˜30 wt % of salt;
- and
- about 5 wt %˜30 wt % of a polyol comprising a copolymer of ethylene oxide and propylene oxide,
- wherein the weight percentage of the salt and the polyol are calculated based on the weight of the extractive solution.
Dependencies are inferred from the claim text.